临床医学论文-白念珠菌基因多态性与耐药性关系.docx

临床医学论文-白念珠菌基因多态性与耐药性关系

作者：黄静，刘慧，朱家馨，周宇麒，谈淑卿，张天托

【摘要】【目的】探讨耐氟康唑白念珠菌基因多态性与其耐药性是否相关。【方法】收集48株敏感白念珠菌和10株耐氟康唑白念珠菌，其中包括2株体外诱导的耐氟康唑白念珠菌。选择1个随机引物(引物1251),朵用任意引物PCR方法基因分型，将电泳图谱扫描入计算机后采用Labwork4.0软件转化为数值图表，利用SPSS11.5进行聚类分析。【结果】引物1251的PCR指纹图带型稳定，多态性丰富，可以作为分型引物·聚类分析结果提示8株临床耐药菌的PCR指纹图相似系数为71.7%,而其中7株的耐药菌相似系数高达86.7%,远高于58株菌的平均相似系数(47.3%)。2株体外诱导的耐药菌诱导前后基因型未发生变化。【结论】基因分型的结果未提示耐氟康唑白念珠菌存在一定的特殊电泳条带，但提示了特定的PCR指纹图可能与耐药性有一定相关性。

【关键词】白念珠菌；基因型；氟康唑；耐药性，任意引物PCR

Abstract:【0bjectives】ToinvestigatethepossiblerelationshipofC.albicansgenepolymorphismsandits

fluconazole-resistance.【Method】Forty-eightfluconazole-susceptiblestainsand10fluconazole-resistantstainsofC.albicanswereanalyzed,withtworesistantstainsinducedinvitro,Allofthemweregenotypedbyarbitraryprimedpolymerasechainreaction(AP-PCR)fingerprintingemployingoneinterrepeatprimes(1251primers).Theimagewascaptured

usingcomputer-assistedsystemwithLabwork4.0softwaretoanalyzethegelpatternsofallisolates.ThedatawereprocessedbySPSS11.5

statisticalsoftware.【Results】Primer1251wassuitablefor

fingerprintinganalysis,andwasfoundtogeneratethereproducible

fingerprintingprofiles,yieldingwel1-resolvedbandingpatterns.TheAP-PCRprofilesof8resistantstainsisolatedclinicallywerehighlysimilaraccordingtosimilaritycoefficient(71.7%).Furthermore,thesimilaritycoefficientof7resistantisolates(86.6%)wassignificantlyhigherthanthatoftotal58stains(47.3%).Fortwoinducedisolates,theirgenotypesdidnotchangemuchafterresistance-inducementinvitro.【Conclusions】NospecificDNAprofilesofC.albicansindicating

fluconazole-resistancewereidentificd.However,ourresults

demonstratedastronggeneticcorrelationbetweenfluconazole-resistantpatternsanditsAP-PCRprofilesinC.albicans.

Keywords:C.albicans;genotype;fluconazole;drug