浅谈土著微生物修复石油污染地下水的研究.docx

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浅谈土著微生物修复石油污染地下水的研究

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摘要：采用优化土著微生物菌群的生物技术，开展了华北濮阳地区石油污染地下水的修复实验。选择优化出的菌群包括假单胞菌属、微球菌属、放线菌属、真菌类的青霉属、曲霉属；实验过程中，分别在石油含量为1820mg/L、8625mg/L、16950mg/L污染水中加入了15%的优化菌群制剂。经过28d、37d、37d的修复实验，三种浓度中石油污染物的最大累计去除率分别为5346%（28d）、7087%（37d）和5819%（37d），和对照5%的净化率相比，加入优化菌群制剂显著提高了石油污染物的去除率；温度是影响修复效果的因素之一，30℃是该菌群的最佳生长温度，对油污地下水的修复效果最明显，浓度是影响石油污染物浓度的另一重要因素。

关键词：土著微生物；修复；石油污染；温度；浓度

1672-1683（2010）06-0056-03ExperimentalStudyonIndigenousMicrobialRemediationofOil-contaminatedGroundwater

CHENLi1，2,ZHANGFa-wang1,ZHANGLin1,ZHANGJuan-juan3

(1.TheInstituteofHydrogeologyandEnvironmentalGeology,ChineseAcademyofGeologicalSciences,Shijiazhuang050061,China;2.ChinaUniversityofGeosciences,BEijing,100083,China;3.ShijiazhuangUniversityofEconomics,Shijiazhuang050000,China)

Abstract:Usingoptimizedindigenousmicrobialflorabiotechnology,wehadconductedrepairexperimentalresearchonoil-contaminatedgroundwaterinPuyangareaofNorthChina.Theselectedoptimizedfloraincludepseudomonas,micrococcus,actinomyces,fungisuchaspenicilliumandaspergillus;inexperimentprocess,add1.5%soptimizedflorapreparationintothepollutedwaterswithoilcontentof182.0mg/l,862.5mg/land1695.0mg/l.After28days，37daysand37daysremediationexperiments,themaximumcumulativeremovalratesofoilpollutantsinthethreeconcentrationsare53.46%(28d),70.87%(37d)and58.19%(37d).Thetemperatureisoneoftheremediationaffectingfactorsand30℃isthebestfloragrowthtemperature,withthemostsignificantremediationeffectonoil-contaminatedgroundwater;concentrationisanotherimportantfactorinfluencingoilpollutants;insterilecontrastexperiment,theremovalrateofoilpollutantsiswithin5%,indicatingslowgroundwateroildegradationinnaturalconditions.

Keywords:microbialremediation;oil;contaminatedgroundwater;temperature;concentration

石油开采过程中井下作业、油气集输、垃圾堆积以及事故等都会造成油类经包气带进入地下水中，危害地下水资源［1-