一种中药组方促进消化功能动物实验研究.docx

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一种中药组方促进消化功能动物实验研究

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摘要：目的研究一种中药组方促进动物消化功能。方法将昆明小鼠随机分为阴性对照组、模型对照组和低、中、高（1.67、3.33、6.67mL/kg·BW）3个受试样品组。受试样品组连续经口给予受试物15d，每天1次；阴性对照组和模型对照组连续给予纯水15d，每天1次。用复方地芬诺酯制造小鼠便秘模型，进行小鼠小肠运动试验，测定小鼠的墨汁推进率。将SD大鼠随机分为阴性对照组和低、中、高（0.83、1.67、3.33mL/kg·BW）3个受试样品组。各剂量组分别连续经口给予受试物30d，每天1次，观察大鼠体重、体重增重、摄食量和食物利用率，测定大鼠的胃液量，胃蛋白酶活性和排出量。结果该中药组方(1.67、3.33、6.67mL/kg·BW)能显著增加小鼠小肠墨汁推进率(P0.01)。中药组方(0.83、1.67、3.33mL/kg·BW)能显著增加大鼠体重增重、摄食量和食物利用率(P0.01或P0.05)。中药组方(1.67、3.33mL/kg·BW)能显著增加大鼠胃液量，胃蛋白酶活性和胃蛋白酶排出量(P0.01或P0.05)。结论本中药组方在研究中发现具有促进消化功能，即能增进食欲和进食量，增加胃液和胃酶分泌量，增加动物体重，同时能够增进小肠蠕动。

关键词中草药组方；消化功能；墨汁推进率；胃蛋白酶

中图分类号R285文献标识码B

ExperimentalstudyonpromotingdigestivefunctionofChildrensLifeOralLiquidinanimals

ZHANGXiuting1,SUNPengpeng2

（1.QiluMedicalUniversity,Zibo255300,China2.ZiboInstitutionforfoodanddrugcontrol,Zibo255000,China）

Abstract:ObjectiveThecurrentstudywasperformedtodeterminetheeffectsofChildLifeOralLiquidonanimaldigestivefunction.MethodsKunmingmicewererandomlypidedintonegativecontrolgroup,modelcontrolgroupaswellasinlow,mediumandhigh(1.67,3.33,6.67ml/kg·BW)threetestsamplegroups.Thetestsamplegroupwasorallyadministeredthetestsubstancefor15days,onceadaywhilethenegativecontrolandthemodelcontrolgroupswerecontinuouslyadministeredpurewaterfor15days,onceaday.Amouseconstipationmodelwasdevelopedusingdiphenoxylatecompoundmoreover,thesmallintestinalmotilitytestwasperformedtodeterminetheinkadvancementrateinintestinaltractofthemice.Furthermore,SDratswererandomlypidedintonegativecontrolgroupalongwithlow,mediumandhigh(0.83,1.67,3.33ml/kg·BW)threetestsamplegroups.Eachdosegroupwasorallyadministeredwiththetestsubstancecontinuouslyfor30days,onceaday.Thebodyweight,weightgain,foodintakeandfoodutilizationrateoftheratswereobserved,inadditionthegastricfluidvolume,peps