mir-96在膀胱尿路上皮癌中的表达及临床意义.docVIP

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mir-96在膀胱尿路上皮癌中的表达及临床意义

mir-96在膀胱尿路上皮癌中的表达及临床意义152JournalofMedica1ScienceinCentralSouthChina,March2012,Vo1.40,No.2文章编号2012临床医学?hsamir一96在膀胱尿路上皮癌中的表达及临床意义汪翼.罗红梅,杨罗艳(1.南华大学附属第二医院泌尿外科,湖南衡阳421001;2.南华大学组胚教研室;3.中南大学湘雅二医院泌尿外科)摘要:目的探讨hsamir-96在膀胱尿路上皮癌的表达及其与膀胱尿路上皮癌病理分级及临床分期的关系.方法采用RealtimePCR方法检测33例膀胱尿路上皮细胞癌及9例癌旁正常膀胱组织中hsamir一96的表达情况,并分析其与膀胱尿路上皮细胞癌病理分级及临床分期的关系.结果hsamir一96在膀胱尿路上皮细胞癌中的表达明显高于癌旁正常膀胱组织(Plt;0.01).其表达与膀胱尿路上皮细胞癌的病理分级,临床分期密切相关,随病理分级,临床分期的增高其表达也升高,但与性别无关.结论hsamir-96可能参与了膀胱尿路上皮细胞癌的发生,发展过程.关键词:hsamir-96;微小RNA;膀胱尿路上皮癌;癌旁正常膀胱组织中图分类号:R737.1文献标识码:AExpressionofhsa--mir?-96inHumanBladderUrotheliumCarcinomaandItsClinicalSignificanceWANGYi,LUOHongmei,YANGLuoyan(DepartmentofUrology,theSecondAffiliatedHospital,UniversityofSouthChina,Hengyang,Hunan421001,China)Abstract:ObjectiveToexploretheexpressionofhsamir一96inhumanbladderurotheliumcarcinomawithtumorgrade.MethodsToevaluatetheexpressionofhsamiR-96inbladderurothelialcarcinoma,RealtimePCRwasperformedin33casesofbladderurothelialcarcinomasand9casesofadjacentnormalbladdertissues.ThecomparativeCt(AACt)methodwasusedtodeterminethefolddifferenceintheexpressionofhsamiR一96indifferentbladderurothelialcarcinomatissuesrelativetotheadjacentnormalbladdertissues.ResultsTheexpressionofhsamiR-96washigherinbladderurothelialcarcinomathaninadjacentnormalbladdertissues(Plt;0.01).Moreover,analysisofhsamiR一96expressionintumorsofdifferentclinicalstagesandpathologicalclassificationdemonstratedthattheexpressionofhsamiR-96washigherinT2一T4stagesthanTaTIstages,andtheexpressionhsamiR一96washigherinpathologicalgradelIIIIthangradeI(Plt;0.01).However,therewasnosignificantdifferencebetweengender(Pgt;0.05).ConclusionsTheseresultsshowedthathsamiR一96mightbeinvolvedintheprocessofoccurrence,development,andinfiltrationofbladderurotheliumcarcinomas.Keywords:hsamiR-96;micoRNA;bladderurotheliumcarcinomas;adjacentnormalbladdertissues近年