基因工程抗体和抗体工程.pptx

基因工程抗体和抗体工程;Cgenescodefortheconstantregionsofimmunoglobulinproteinchains.

Vgeneissequencecodingforthemajorpartofthevariable(N-terminal)regionofanimmunoglobulinchain.;Figure24.4Heavyandlightchainscombinetogenerateanimmunoglobulinwithseveraldiscretedomains.;Figure24.4Heavyandlightchainscombinetogenerateanimmunoglobulinwithseveraldiscretedomains.;Table24.1EachimmunoglobulinfamilyconsistsofaclusterofVgeneslinkedtoitsCgene(s).;Figure24.5ThelambdaCgenesegmentisprecededbyaJsegment,sothatV-Jrecombinationgeneratesafunctionallambdalight-chaingene.;Figure24.6ThekappaCgenesegmentisprecededbymultipleJsegmentsinthegermline.V-JjoiningmayrecognizeanyoneoftheJsegments,whichisthensplicedtotheCgenesegmentduringRNAprocessing.;Figure24.7Heavygenesareassembledbysequentialjoiningreactions.FirstaDsegmentisjoinedtoaJsegment;thenaVgenesegmentisjoinedtotheDsegment.;Figure24.8ThelambdafamilyconsistsofVgenesegmentslinkedtoasmallnumberofJ-Cgenesegments.;Figure24.9ThehumanandmousekappafamiliesconsistofVgenesegmentslinkedto5JsegmentsconnectedtoasingleCgenesegment.;Figure24.10Asinglegeneclusterinmancontainsalltheinformationforheavy-chaingeneassembly.;Figure24.12Consensussequencesarepresentininvertedorientationateachpairofrecombiningsites.Onememberofeachpairhasaspacingof12bpbetweenitscomponents;theotherhas23bpspacing.;Figure24.13Breakageandreunionatconsensussequencesgeneratesimmunoglobulingenes.;Figure15.8Reciprocalrecombinationbetweendirectrepeatsexcisesthematerialbetweenthem;eachproductofrecombinationhasonecopyofthedirectrepeat.;Figure15.9Reciprocalrecombinationbetweeninvertedrepeatsinvertstheregionbetweenthem.;一、噬菌体抗体库技术

基础方法;它是在PCR技术和PhageDisplay基础上实现。其过程是把用PCR法得到抗体基因插入丝状噬菌体DNA，与噬菌体外壳蛋白基因相连，在辅助噬菌体帮助下，噬菌