PCR检测时对实验室DNA污染的清除.docx

紫外线照耀去除HBV－DNA作用争论刘军权

【摘要】目的观看紫外线对PCR外源性HBV－DNA污染的作用。方法用紫外线对不同浓度的HBV－DNA阳性血清标本和HBV阳性参控品进展固相和液相状态紫外线照耀。结果三种枯燥后的标本随着紫外线照耀时间的延长，HBV－DNA去除率明显增加。HBV阳性参控品与一样HBV－DNA拷贝数的血清标本置紫外线照耀后，每一样的照耀时间HBV－DNA下降率前者均显著高于后者。血清标本和HBV阳性参控品经1∶1万倍稀释、紫外线照耀2小时后，前者HBV

－DNA下降33.3倍，后者下降6250倍。结论紫外线消毒法对阳性血清标本造成的试验室污染不能去除；阳性模板经紫外线消照耀后HBV－DNA含量明显降低；紫外线照耀对去除物体外表较低含量阳性HBV－DNA模板或HBV－DNA引起的气溶胶可能有效。

【要害词】紫外线消毒聚合酶链反响乙型肝炎病毒

StudyonClearedeffectofUltravioletradiationininducedcontaminationofHBV

－DNA

LIUJunquan,ZHOUZhonghai,YANWeimin.DepartmentofCentralLaboratory,97thhospitalofPLA,Xuzhou221004,P.RChina

【Abstract】ObjectiveToobservetheeffectsofUltravioletradiationoncontaminationofexternalHBV－DNA.MethodsHBV－DNApositiveserumsamplesandHBVpositivecontrolserumsampleswithdifferentconcentrationweredisinfectedbyUltravioletradiationinsolidstateandliquidstate.ResultTheclearedrateofHBV－DNAinthreekindsofdrysamplesincreasedmarkedlyalongwithprolongeddisinfectanttimeofUltravioletradiation.AfterHBVpositivecontrolsamplesandserumsampleswithsamecopieshavebeenirradiatedbyUltravioletradiation,it

showedthattheclearedrateofHB－VDNAoftheformerwasremarkablyhigherthan

thatofthelatter.HBV－DNAofserumsamplesreduced33.3-foldandthatofHBVpositivecontrolsamplesreduced6250-foldaftertheyweredilutedto1:10000anddisinfected2hoursbyUltravioletradiation.ConclusionThepositiveserumsamplesinducedcontaminationinlaboratorycouldnotbeenclearedawaybyUltravioletradiation;Beendisinfected,theconcentrationofHBV－DNAofpositiveserumsamplesreducedobviously;UltravioletradiationmayhaveeffectsinclearingawaythelowconcentrationofHBV－DNAofpositiveserumsamplesoraerosolinducedbyDNAon

surfaceofobjects.

【Keywords】Ultravioletradiation;disinfect;Polymerasechainreaction;HepatitisBvirus

紫外线照耀是聚合酶链反响〔PCR〕测定的试验室常用消毒方法，对掌握外源性DNA污染引起的假阳性较