海洋真菌Phaeosphaeriopsis sp. ZYX-Z-811次生代谢产物研究.pdfVIP

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海洋真菌Phaeosphaeriopsissp.ZYX-Z-811次生代谢产物研究

摘要:对海洋真菌Phaeosphaeriopsissp.ZYX-Z-811发酵液中次生代谢产物进行分离鉴定,并评价其生物活性。采用正相硅

胶柱色谱、反向C18柱色谱、高效液相色谱、SephadexLH-20凝胶柱色谱等技术进行分离纯化,共分离得到10个化合物,利

用核磁及红外和紫外波谱技术对分离的单体化合物进行结构鉴定,分别为4-epi-lignicol(1)、lignicol(2)、6-demethylkigelin

(3)、6,8-dihydroxy-3,4,7-trimethylisocoumarin(4)、6,7-dimethoxymellein(5)、musaoneA(6)、2-(4-hydroxyphenyl)ethylacetate

(7)、3-吲哚甲醛(8)、吲哚-3-乙酸甲酯(9)、对羟基苯甲酸甲酯(10),其中化合物1为未发表的化合物,文章通过电子圆二色

谱(electrostaticcirculardichroism,ECD)计算确定了其绝对构型。对这些化合物分别进行抗氧化和α-糖苷酶抑制活性评价,发

现化合物10有弱的抗氧化活性。

关键词:海洋真菌;Phaeosphaeriopsissp.;次生代谢产物;生物活性

StudyonthesecondarymetabolitesofmarinefungusPhaeospheriopsissp.ZYX-Z-811

Abstract:ThesecondarymetabolitesofmarinefungusPhaeosporiopsissp.ZYX-Z-811wereisolatedandidentified,andtheir

biologicalactivitieswereevaluated.ThecompoundswereseparatedandpurifiedbySilicagel,C18,SephadexLH-20column

chromatographyandhighperformanceliquidchromatography(HPLC),tencompoundswereseparated,andtheirstructureswere

identifiedbyNMR,IRandUVspectroscopicmethods.Tencompoundswereidentifiedas4-epi-lignicol(1),lignicol(2),6-

demethylkigelin(3),6,8-dihydroxy-3,4,7-trimethylisocoumarin(4),6,7-dimethoxymellein(5),musaoneA(6),2-(4-

hydroxyphenyl)ethylacetate(7),3-indoleformaldehyde(8),indole-3-aceticacidmethylester(9),methylp-hydroxybenzoate(10),

amongwhichcompound1wasnotpublishedinjournal,andtheabsoluteconfigurationwasdeterminedbyECDcalculation.These

compoundswereevaluatedforantioxid