红酵母中提取酵母多糖的提取工艺论文.doc

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本科生毕业论文

红酵母中提取酵母多糖的提取工艺

摘要

本实验采用的是CICC编号31229的红酵母，通过对红酵母的实验，了解红酵母的特性及生长特点，进而改善红酵母的培养条件及培养基来扩大红酵母的生长浓度。研究从红酵母中提取酵母多糖的提取工艺，确定酵母多糖提取方法。

采用摇瓶培养的方法，通过碳源、氮源及主要营养与环境条件等对红酵母产菌量的影响试验，以及葡萄糖、蛋白胨、酵母膏三因素三水平的正交试验，获得了红酵母的最佳生长条件：葡萄糖2％，蛋白胨1．0％，酵母膏0,5％，磷酸二氢钾0．15％，硫酸镁0．01％，氯化钠1．0％，初始pH5．0，接种量10％，500mL三角瓶中培养基装量为80mL，发酵温度为28℃，摇床200r／min。在此优化的培养条件下，海洋红酵母的产菌量可达9．7亿个／mL，比优化前发酵培养基产菌量（5．6亿个／mL）提高了73．2％，比单因素试验结果（8．1亿个／mL）提高了19．8％。

研究从酵母中提取胞壁多糖的提取工艺。提取工艺路线为：酵母溶解—冻融一超声波破碎一碱溶一中和一沉淀一洗涤—烘干。通过正交试验对酵母破壁和碱溶条件进行优化。初步寻求提取工艺条件，多糖得率为19．4％，用苯酚一硫酸法测定多糖的含量为51．9％。

关键词:红酵母高细胞密度培养条件优化酵母多糖提取工艺

Abstract

TheexperimentwasthroughthestudyoftheRhodotorulaglutinis，tounderstandthecharacteristicsandgrowthcharacteristicsofRhodotorulaglutinis,Therebyimprovingconditionsforthecultivationofredyeastandthemediumtoexpandthegrowthofredyeastconcentration.

Aflaskoftrainingmethods，TheuseofRhodotorulaglutinis，Throughthecarbonsource,thenitrogensourceandthemainnutritionandtheenvironmentalconditionandsoonproducethefungusquantitytotheRhodotorulaglutinisbenthicatheinfluenceexperiment,aswellasthesucrose,theproteinpeptone,theyeastpastethreefactorsthreelevelsorthogonalexperiments,wehaveobtainedtheRhodotorula＇sbestfermentationcondition：sucrose1.5%,proteinpeptone1.0%,yeastpaste0.5%,potassiumdihydrogenphosphate0.15%,magnesiumsulfate0.01%,sodiumchloride1.0%,initialpH5.0,inoculatingquantity8%,80mLmediuminthe500mLtrianglebottle,temperature28℃

Thisexperimentisstudyingonthecraftofpolysaccharidesextractionfromrhodotorulcell-wal1．Thecraftroutedrawn：yeastdissolve—freezethawing—uhrasoinccrush—alkaliabstraction—neutralize—deposit—solventwash—dry．Seekthebestcraftcondition，aswellasoptimizeconditionsofcrushwallandalkalidissolvingbyorthogonalexperiment．yieldofpolysaccharideis19.4％．Determinationwithphenol—sulphuricaicdlaw．contentofpolys