生物化学与分子生物学PPT图片素材.ppt

生物化学与分子生物学

PPT素材可根据需要选择取舍

Metabolismof5-fluorouracil(5-FU)5-FU

Carac,Efudex5-Fluorouridine5-FUMP5-FdUMPUridine

phosphorylase5-FluorodeoxyuridineThymidine

phosphorylaseOrotate

phosphoribosyl

transferaseThymidine

kinase5-FUTP5-FdUTPRNADNA5’-nucleotidase5’-nucleotidaseUridine

kinaseATPADPATPADPPRPP

WesternBlotting1.Resolveproteinsamples

onSDS.Incubatetheblotwith

chemiluminescentHRP

substrateandexposetofilm2.Electrophoreticallytransfer

fractionatedproteinsfrom

gelontoPVDFmembrane3.Blockthemembrane

withneutralprotein

(BSAormilkcasein)4.Incubatethemembrane

withprimaryantibody

specifictotargetprotein5.Incubatethemembrane

withHRP-labeledsecondary

antibodyspecifictoprimary

antibody

CholesterolSterolToolkitPregnenolonProgesteronAldosteronCortisolCorticosteroneAndrostendioneTestosteronEstriol17?-Estradiol1,25-DihydroxycholecalciferolCholicacid

Covalentmodificationofproteins

Poly(ADPribosyl)Polymerase(PARP)Poly(ADPribosyl)

polymerase

(PARP)NADNicotinamidePoly(ADPribosyl)

polymerase

(PARP)Poly(ADPribosyl)

chainelongation

andbranchingNicotinamideNADNADDNApolymerase?DNApolymerase?DNApolymerase?

Oligosacharyl

transferaseN-glycosylationpathwayinERMann-P-DolicholtransferasesFlippaseDolicholPDolicholPPDolicholPPDolicholPPDolicholPPGlcNAc-P

transferaseGlcNAc

transferaseDolicholPDolicholPPDolicholPPGDP-Mannosyl

transferasesGlc-P-Dolichol

transferasesDolicholPDolichol-pyrophofphataseCytosolER40S60S

UTPCTPTMPPyrimidineNucleotideInterconversionsUMPATPADPH2OUDPNucleoside

monophosphate

kinaseATPADPNucleoside

diphosphate

kinaseATPADPCTP

synthaseRibonucleotide

reductasedeoxyUDPNucleoside

diphosphate

phosphohydrolase

deoxyUMPThymidylate

synthaseN5,N10-MethyleneTHFDHFTTPNucleoside

monophosphate

kinaseNucleoside

diphosphate

kinase

Myo-Inositol-3-phosphate

Ins(3)P1BiosynthesisandmetabolismofphosphoinositidesGlucose6-pgosphateIns(3)P1

synthaseIns(3)P1

phosphatas