骨髓细胞学检查课件.pptxVIP

骨髓细胞学检查 contents ØThe developmental sequence of marrow cells Ø Indications and contraindications of bone marrow examination Ø Specimens collection Ø Steps of bone marrow examination Ø Report and evaluation of bone marrow examination The developmental sequence of marrow cells Lymphoid Lymphoid ~ Lymphocytes stem cells Erythroid ~ Erythrocytes Myelomonocytic ~ Neutrophils/monocytes Bone Marrow Stem Cells Progenitor cells Eosinophils Basophils Megakaryocytes Platelets Blood Mature cells Eosinophil ~ Basophil ~ Myeloid stem cells T S C Myeloid Eosinophil ~ Eosinophils 2~4:1 N N normal bone marrow Morphologic examination of marrow cells 2~4:1 N N normal bone marrow normoblast basophilic polychromatic orthochromatic erythrocyte cells divided by the percentage of The developmental sequence of marrow cells Morphologic examination of marrow cells Myeloid series:Erythroid series 2~4:1 ↑ ↑ erythroleukemia Lymphocytes ： 20% of nucleated cells polychromatic normoblasts ＜10% Assesment the degrees of marrow hyperplasia Observe abnormal cells ＜2:1 ↓ N agranulocytosis myeloblast promyelocyte myelocyte metamyelocyte stab segmented 嗜酸性 嗜碱性 原单核细胞 幼单核 单核细胞 原巨核细胞 幼巨核细胞 颗粒型巨核细胞 产血小板型巨核细胞 血小板 原淋巴细胞 幼淋巴细胞 淋巴细胞 Development of progenitor cells 中幼红细胞 晚幼红细胞 红细胞 polychromatic orthochromatic erythrocyte normoblast normoblast 中幼粒细胞 晚幼粒细胞 杆状核 分叶核 早幼红细胞 basophilic normoblast 早幼粒细胞 原红细胞 normoblast 原粒细胞 Indications of bone marrow examination Ø Diagnose hematologic diseases -Definitive diagnosis： leukemia， multiple myeloma(MM) -Helpful diagnosis