文稿分析案例教案part i structural biochemistry上传版chapter4protein properties classification and research methods.pptx

Chapter 4 Protein Properties, Classification Research Methods;Outline;Some Properties of Proteins;Denaturation;Loss of Biological Activity Loss of Solubility Increased Proteolysis More sensitive to protease Increased viscosity ;Protein Hydrolysis;Enzymatic Hydrolysis;Specificities of Various Endopeptidases;Experimenting with proteins…;1. How big is protein X?;Electrophoresis;Electrophoresis;2. What is protein Xs isoelectric point?;Procedure: Make a gel with pH gradient Load protein Find the pH where the protein stops moving;If you combine SDSand isoelectric focusing, you get 2-dimensional gel electrophoresis (2D gel);3. Is protein X found in other cell?;With the help of antibodies!;How are antibodies against protein X obtained?;Detection of Protein X;4. What is the sequence of protein X?;Overview of protein sequencing (1);Overview of protein sequencing (2);5. What is the tertiary structure of protein X?