EGCG磷脂复合物抗氧化活性的研究.PDF

现代食品科技 Modern Food Science and Technology 2015, Vol.31, No.5 EGCG 磷脂复合物抗氧化活性的研究 陈金玉，张鑫，吴祖芳，翁佩芳 （宁波大学海洋学院，浙江宁波315211) 摘要：本文研究了表没食子儿茶素没食子酸酯磷脂复合物的体外抗氧化活性。将EGCG 和大豆卵磷脂溶于无水乙醇，制备得到 EGCG 磷脂复合物。通过高效液相色谱(HPLC)测定，发现制备得到的EGCG 磷脂复合物中EGCG 的复合率大于99%，并利用紫外扫 描、红外光谱、X 射线衍射等方法对其结构进行表征，说明通过本实验方法制备得到了目标复合物。通过DPPH、FRAP、TEAC 等 方法测定EGCG 磷脂复合物的体外抗氧化活性，结果表明EGCG 磷脂复合物的体外抗氧化能力显著高于VE （P0.05 ）。同时测定添 加EGCG 磷脂复合物后，不同贮藏时间（3、6、9、12 和15 d ）反映大豆油氧化的指标。结果表明EGCG 磷脂复合物对于大豆油的 氧化具有抑制作用且呈现剂量依赖关系，并且在贮藏 15 d 时，抗氧化效果显著好于BHT （P0.05 ）。研究表明，制备得到的EGCG 磷脂复合物具有显著的抗氧化活性，并且可以有效抑制大豆油的氧化。 关键词：EGCG；EGCG 磷脂复合物；抗氧化；大豆油 文章篇号：1673-9078(2015)5-137-143 DOI: 10.13982/j.mfst.1673-9078.2015.5.022 Antioxidant Activity of (-)-Epigallocatechin Gallate-phospholipid Complex CHEN Jin-yu, ZHANG Xin, WU Zu-fang, WENG Pei-fang (Department of Food Science, School of Marine Sciences, Ningbo University, Ningbo 315211, China) Abstract: The in vitro antioxidant activity of (-)-epigallocatechin gallate (EGCG)-phospholipid complex (EPC) was studied. First, EPC was prepared by dissolving EGCG and soybean lecithin in ethanol. High-performance liquid chromatography showed that the complexation rate of EGCG in EPC was more than 99%, and the structure of EPC was characterized by ultraviolet spectroscopy, infrared spectroscopy, and X-ray powder diffractometry. The in vitro antioxidant activity of EPC, as evaluated by diphenylpicrylhydrazyl and ferric reducing ability of plasma and Trolox equivalent antioxidant capacity methods, was found to be significantly higher (p 0.05) than that of VE. Indicators reflecting the oxidation of soybean oil at different storage times (3, 6, 9, 12, and 15 days) were also evaluated after the addition of EPC. The results showed that