药物分析学 第三次作业.docxVIP

《药物分析学》第三次作业 徐韵韶找一篇关于【固相微萃取】的文献，翻译【生物样品预处理】部分内容。 文献信息 生物样品预处理内容 SPME技术集采样、萃取、浓缩、进样于一体，已成为复杂基体中痕量组分分析的主流提取分析方法。本实验采用顶空（head space，HS）-SPME-GC-MS联用技术对库尔勒香梨不同花期花香成分进行研究，优化了HS-SPME条件参数，建立了鉴定库尔勒香梨花序香气成分种类及含量的GC-MS方法，为进一步研究库尔勒香梨花香气生理生态功能，以及研究库尔勒香梨花香气与果香气的关系等提供一定方法依据和数据支持。供试材料为库尔勒市沙依东园艺场梨园健康植株（树龄15 a左右）花蕾期、盛花期完整花序，现场称量，迅速剪碎、充分混匀后分别装入15 mL顶空瓶，拧紧密封盖，备用。 将SPME针插入GC-MS进样口，250 ℃条件下老化5 min。若首次使用，则需将老化时间延长至30 min。将SPME萃取纤维头手动进样器插入已称取好完整花序的顶空萃取瓶中，将整个萃取设备放入一定温度水浴锅中顶空吸附一段时间后抽回，然后将SPME萃取纤维头插入GC-MS进样口250 ℃，解吸5 min。 翻译 SPME technology includes sampling, extraction, concentration, injection, which has become the mainstream in the analysis of trace components in complex matrices analysis. In this study, headspace (head space, HS) -SPME-GC-MS combined technique on Korla Pear Flowering floral constituents of optimized parameters of HS-SPME conditions established identification Korla Pear aromatic components and inflorescence content of GC-MS method for further study Korla fragrant pear aroma physiological and ecological functions, and the relationship Korla fragrant pear aroma fruit aroma and the like provide a method basis and data support. Tested materials Korla pear sand by East market gardening healthy plants (about age 15 a) bud stage, flowering inflorescences complete, on-site weighing and quickly cut into pieces, respectively, into 15 mL vial mix well, tighten closures, alternate. The SPME needle into GC-MS Inlet, 5 min at 250 ℃ aging conditions. If the first time, you need to set the aging time was extended to 30 min. The SPME fiber extraction head manually inserted into the injector has weighed good complete inflorescence headspace extraction flask, the entire extraction device in a certain temperature water bath headspace adsorption after a period of time to withdraw, then extracted SPME fiber insertion head GC-MS inlet 250 ℃, and desorbing 5 min.