急性肝功能衰竭相关基因和蛋白的研究-生物学、细胞生物学专业论文.docx

AB AB STRACT Acute hepatic failure(AHF)is kind of liver disease with high mortality rate caused by vmet=), of factors and seriously threatens people’s health．It severely damages the liver cell and leads to dysfunction of synthesis，detoxification and biotransformation of the liver．AHF happens often accompanied by multiple organ failure，coagulation disorders，hepatic encephalopathy,secondary infection， hemodynamic disorders and metabolic complications．It is not fully understood about the pathogenesis and molecular mechanism ofAHF because of the complexity due to numerous factors．So，current treahnent to AHF is mainly focused the comprehensive treatment due to the lack of effective control measures．For this reason，AHF has been of the most challenging diseases clinical medicine． In this paper,we successfully constructed the AHF rat model and tested the modelling effect by detecting the enzymatic activity of senlm alanine transaminase(ALT)and aspartate aminotransferase (AST)，and counting the coefficient of liver and observing of frozen sections under microscopic．We detected gene expression profile and found 48 1 9 genes associated with AHF utilizing the Rat Genome 230 2．0 Gene Chip(RGC)．We uploaded the data to Ingenuity Pathway Analysis(IPA)and found that IL-l， IL-6 and IL-8 signaling pathway signaling activated and p53，ATM and AMPK signaling pathway decreased．Cell survival，proliferation and differentiation activated，at the$anle time，the apoptosis ofT and B lymphocytes decreased．ARer the analysis of the gene expression related to AHF we predict that Ⅱ，ln_lLlRl-+_MAPK8_+FOS，H，N and／or TNFa*TNFRSFlA／B--,--,Caspases pathway regulate apoptosis at injury and progressing stage，inflammatory factors regulate apoptosis by NF·rB pathway at progressing stage，TP53 inhibits apoptosis砒progressing stage,apoptotic is inhibited at recovery stage due IlI 万方数据 to to the down-regulation of Caspase3 and the injury was repaired by proliferation at progressing and recovery stage due to the up-