细菌启动子识别及应用与研究进展.pdf

生 物 工 程 学 报 Chin J Biotech 2010, October 25; 26(10): 1393−1403 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn ©2010 CJB, All rights reserved. 细胞工厂的构建技术 细菌启动子识别及应用研究进展 1 1 2 2 徐友强 ，马翠卿 ，陶飞 ，许平 1 250100 2 200240 摘 要: 细菌启动子是细菌中基因表达的必需调控元件，决定了细菌基因表达的强度和时机。通过启动子的插入或缺 失，可以改变细菌基因的表达，实现对菌体生长发育以及代谢调控的研究。启动子也是构建各种表达系统、实现异源 基因表达的基础。启动子的识别和应用研究，对于实现异源基因的可控表达、有效获得目的产物、促进生物催化和代 谢工程研究具有重要的意义。以下对细菌启动子进行了简单的介绍，总结了细菌启动子的识别方法，并对细菌启动子 的研究进展和具体应用进行了概述。 关键词: 启动子识别，诱导型启动子，启动子应用 Bacterial promoter recognition and application 1 1 2 2 Youqiang Xu , Cuiqing Ma , Fei Tao , and Ping Xu 1 State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China 2 MOE Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China Abstract: Bacterial promoter is a kind of regulators which are needed in bacterial gene expression and decide the strength and opportunity of gene expression. By insertion or deletion of promoters, we can change bacterial gene expression in order to study the growth and metabolic regulation. Promoters are also used to construct many kinds of vectors, so as to express heterologous genes. The study of promoter recognition and application is of great importance to realize the regulation of genes, gain products effectively and promote biological catalysis and metabolic engineering. This paper reviews bacterial promoters, and the methods for recognition of bacterial promoters as well as the study and application of bacterial promoters. Keywords: promoter recognition, inducible promoter, promoter application