Th17细胞及相关因子在多发性硬化中表达变化及临床意义.DOC

Th17细胞及相关因子在多发性硬化中表达变化及临床意义* 刘博文1，谭戈2 （1重庆医科大学附属第一医院金山医院内科 401122; 2重庆医科大学附属第一医院神经内科 400016） 摘要：目的 探讨多发性硬化患者体内Th17细胞及相关细胞因子表达的变化及临床意义。 方法 收集多发性硬化患者27例（MS组）和健康对照20例（对照组）。采用RT-PCR检测外周血单个核细胞（PBMCs）中IL-17m RNA的表达；采用酶联免疫法（ELISA）检测IL-17、IL-23和IL-6水平；采用流式细胞术检测Th17细胞的表达。结果 MS组外周血PBMCs中IL-17 mRNA的表达显著高于对照组（p0.05）；MS组外周血中Th17细胞及相关细胞因子IL-17、IL-23和IL-6的表达也显著高于对照组（p0.05）。结论Th17细胞及其相关细胞因子可能参与了多发性硬化的发生与进展，检测Th17细胞对诊断多发性硬化具有一定的临床意义。 关键词：Th17细胞；白介素17；多发性硬化 中图分类号：R733.3 文献标识码：A Expression Change of Th17 Cells and Related Cytokines in Multiple Sclerosis and Its Clinical Significance LIU Bo-wen1，TAN Ge2 (1 Jinshan hospital of The First Affiliated Hospital of Chongqing Medical University, Chongqing,401122,China,2 The First Affiliated Hospital of Chongqing Medical University, Chongqing,400016,China) Abstract: [Objective] To investigate the expression of change of Th17 cells and related cytokines in multiple sclerosis patients and its clinical significance.[ Methods] 27 multiple sclerosis patients (MS group) and 20 healthy subjects (Control group) were selected. RT-PCR was used to detect IL-17 mRNA, ELISA was used to detect IL-17, IL-23 and IL-6; FACS was used to detect Th17 cells in peripheral blood. [Results] Level of IL-17 mRNA was significantly increased in MS group (p0.05). When compared to control group, expression of Th17 cells and related cytokines (IL-17, IL-23 and IL-6) was significantly increased in MS group (p0.05). [Conclusions] Th17 cells and related cytokines may participate in the pathogenesis of multiple sclerosis. Detection of Th17 cells could have clinical value in diagnosis of multiple sclerosis. Key words: Th17 cells；IL-17；multiple sclerosis *基金项目：国家自然科学基金资助项目（NO；国家临床重点专科建设项目资助(卫办医政函[2012]649号) [通信作者]谭戈，E-mail： HYPERLINK mailto:titot@ titot@；Tel：023多发性硬化（Multiple sclerosis，MS）)是一种慢性自身免疫性疾病，以中枢神经系统炎症性脱髓鞘和脑血管周围炎性细胞浸润为主要特征[1]。目前尚不明确MS确切的发病机制，但已有研究证实，在MS的发病和病情进展中CD4+ T细胞起着重要的介导作用[2]。Th17细胞是近年来发现的一种新型CD4+效应性T细