荧光分析描述方法好文章Hydrogenperoxideproductionisanearlyeventduring(1).pdf

Planta (2007) 225:1421–1429 DOI 10.1007/s00425-006-0450-6 ORIGINAL ARTICLE Hydrogen peroxide production is an early event during bicarbonate induced stomatal closure in abaxial epidermis of Arabidopsis V. A. Kolla · A. Vavasseur · A. S. Raghavendra Received: 16 July 2006 / Accepted: 6 November 2006 / Published online: 8 December 2006 © Springer-Verlag 2006 Abstract The presence of 2 mM bicarbonate in the Abbreviations incubation medium induced stomatal closure in abaxial ABA Abscisic acid epidermis of Arabidopsis. Exposure to 2 mM bicarbon- DPI Diphenylene iodonium ate elevated the levels of H2O2 in guard cells within H2DCF-DA DichloroXuorescein diacetate 5 min, as indicated by the Xuorescent probe, dichloro- MJ Methyl jasmonate Xuorescein diacetate (H2DCF-DA). Bicarbonate- PI3P Phosphatidylinositol 3-phosphate induced stomatal closure as well as H2O2 production ROS Reactive oxygen species were restricted by exogenous catalase or diphenylene iodonium (DPI, an inhibitor of NAD(P)H oxidase). The reduced sensitivity of stomata to bicarbonate and Introduction H2O2 production in homozygous atrbohD/F double mutant of Arabidopsis conWrmed that NADP(H) oxi- Stomatal movements are brought out through volume dase is involved during bicarbonate induced ROS pro- changes in guard cells in response to various factors duction in guard cells. The production of H2O2 was such as light, temperature, CO2, or phytohormones quicker and greater with ABA than that with bicarbon- (Willmer and Fricker 1996). The stomatal opening is ate. Such pattern of H2O2 production may be one of stimulated by ligh