毛囊隆凸部细胞分离培养和性能.pdf

Colloids and Surfaces B: Biointerfaces 47 (2006) 50–56 Bulge cells of human hair follicles: segregation, cultivation and properties Yi Zhang b,1, Mingming Xiang a , Yun Wangb , Jun Yanb , Yijun Zengb , Jin Yub , Tian Yangb,∗ a Department of Dermatology of Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China b Department of Cell Biology, Third Military Medical University, Chongqing 400038, PR China Received 19 April 2005; received in revised form 12 October 2005; accepted 21 November 2005 Available online 4 January 2006 Abstract The bulge region of hair follicle has been reported as a putative reservoir of hair follicle stem cell (HFSC) for years; however, few studies were done about the characteristics of bulge-originated cells in vitro up to now. In this experiment, the bulge cells isolated from human hair follicles by enzymatic digestion and microdissection were cultured and passaged, and the morphological and biological features of cultured bulge cells were investigated by microscopy and immunocytochemistry. The result showed that new-proliferated cells could be observed on the second day after inoculation, and the quantity of the cells with a greater proliferation potential, reached a peak at the 6th day and maintained this higher level for several days. The mitotic figures of bulge cells were seen and these cells showed undifferentiated morphologic features. The bulge cells strongly expressed K19 and 1-integrin, which are the markers of HFSC, in a descensive way with the culture time. The result indicates that the cultured bulge cell from human hair follicle possesses the properties of primitive cells and supports the hypothesis that HFSC resides in the bulge area. © 2005 Elsevier B.V. All rights reserved. Ke