沙蒿籽胶多糖的分级纯化和结构分析-北京交通大学学报.PDF

※基础研究 食品科学 2007, Vol. 28, No. 03 73 1 2 刘敦华 ，谷文英 (1.宁夏大学农学院，宁夏 银川 750011；2.江南大学食品学院，江苏 无锡 214036) ： 采用0.1mol/L的氢氧化钾对沙蒿籽胶多糖进行组分分级得到水溶多糖(AG1)和水不溶多糖(AG2)。利用离 子交换柱层析从AG1 中分离得到酸性多糖(AG11)和中性多糖(AG12)。经凝胶过滤色谱检测，AG11 和AG12 为均 一组分。化学组成分析表明，AG1 为葡萄糖糖基组成的葡聚糖，并含有糖醛酸；AG11 和AG12 分别为阿拉伯糖、 鼠李糖、木糖、甘露糖、葡萄糖和半乳糖等糖基组成的杂多糖，并含有糖醛酸，相对分子质量分别为8.63×105Da 和3.46 ×105Da。红外光谱测定表明，AG11、AG12 和AG2 具有多糖的特征吸收，并且其糖环均为吡喃环。 ：沙蒿籽胶；分级；纯化；结构 Fractionation and Structure of Polysaccharides of Artemisia sphaerocephala Krasch gum 1 2 LIU Dun-hua ，GU Wen-ying (1.Agricultural College, Ningxia University, Yinchuan 750011, China； 2.School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China) Abstract ：The polysaccharides of Artemisia sphaerocephala Krasch gum were graded into water-soluble polysaccharide (AG1) and water-insoluble polysaccharide (AG2) with 0.1mol/L NaOH AG11 and AG12 were obtained with ion-exchange method from AG1, shown to be homogeneous by gel filtration chromatography. AG2 contains glucose predominantly, and also uronic acid. AG11 and AG12 contains arabinose, rhamnose, xylose, mannose, glucose and galactose predominantly and respectively, and 5 5 also uronic acid. The molecular weights of AG11 and AG12 are 8.63×10 Da and 3.46×10 Da respectively. The structure of AG11, AG12 and AG2 contains pyranride and has the characteristic of glycoconjugate. Artemisia sphaerocephala Krasch gum；fractionation；purification；structure Key words： 中图分类号：Q946.3