鸟嘌呤核苷交换因子Dock180与Elmo1在卵巢癌细胞中的共表达.doc

鸟嘌呤核苷交换因子Dock180与Elmo1在卵巢癌细胞中的共表达 张 瑜1，高一萌1，李文燕1，彭慧娟1，刘 斌2，王 辉1，令狐华1（400016重庆，重庆医科大学：附属第一医院妇产科1，基础医学院病理科） 【摘要】 目的 寻找卵巢癌细胞中鸟嘌呤核苷交换因子Dock180与Elmo1呈相互协同作用的依据。方法 采用 Western blot检测人卵巢癌组织、良性肿瘤组织及正常卵巢组织中的Dock180与Elmo1的表达水平；免疫组化检测卵巢癌组织中Dock180与Elmo1的的分布；免疫荧光检测Dock180与Elmo1在卵巢癌细胞SKOV3中的定位分布；检测Dock180表达缺失的SKOV3细胞中内源性Elmo1的表达水平。结果请补充关键结果的具体数据 Dock180与Elmo1在人卵巢癌组织中的表达水平呈明显正相关（Sperman相关系数r=0.829，P0.05），在卵巢癌组织中二者的表达水平显著高于卵巢良性肿瘤组织组和卵巢正常组织组（P0.05）（Dock180:1.054±0.114、0.518±0.126、0.425±0.072;Elmo1: 0.864±0.114、0.374±0.076、0.300±0.105）。免疫组化及细胞化学染色均显示二者在人卵巢癌组织和细胞内的分布具有一致性。而且在Dock180表达缺失细胞中，Elmo1的表达水平也同时降低。结论 上述结果提示Dock180与Elmo1在上皮性卵巢癌中的表达及分布具有一致性，二者在卵巢癌的癌变过程中可能具有相互协同作用。 请补充关键结果的具体数据 [关键词] 鸟嘌呤核苷交换因子；Dock180； Elmo1； 卵巢癌 [中图法分类号] R737.31; R394.3; R730.23；R711.75 [文献标志码] A Co-expression of Guanine-nucleotide Exchange Factor DOCK180 and ELMO1 in Ovarian Cancer Cells Zhang Yu1, Gao Yimeng1,Li Wenyan1, Peng Huijuan1，Liu Bin2, Wang Hui1, Linghu Hua1 (Department of Obstetrics and Gynecology，First Affiliated Hospital1, Department of Pathology, Basic Medical College2,Chongqing Medical University, Chongqing, 400016, China) ［Abstract］ Objective To seek evidence to support the synergisitic effect between Guanine-nucleotide exchange factor (GEFs) Dock180 and Elmo1 in ovarian cancer cells . Methods Dock180 and Elmo1 expression were detected by Western blotting in ovarian cancer tissues ( EOC，n = 29) ，benign ovarian tumors ( BOT，n = 14) and normal ovary tissues ( Normal，n = 10) . Immunohistochemistry was performed to detect the distribution of Dock180 and Elmo1 in ovarian cancer tissues. Immunofluorescence was utilized to detect the location and distribution of Dock180 and Elmo1 in ovarian cancer cell SKOV3. And finally, endogenous Elmo1 expression was assayed in Dock180 knockdown cell, which was established previously. Results 同中文修改Both Dock180 and Elmo1 expression present with higher level in EOC than those in benign and normal ovarian tissues( P ＜ 0. 05), and no significant difference w