《瑞香狼毒(Stellera_chamaejasme_l.)根乙酸乙酯萃取物化学成分的分离与提纯》-毕业论文（设计）.docVIP

瑞香狼毒(Stellera chamaejasme l.)根乙酸乙酯萃取物化学成分的分离与提纯 摘要：对瑞香狼毒根乙酸乙酯萃取物的化学成分及杀螨活性进行研究，寻找植物源杀螨剂先导化合物。用各种色谱方法分离、纯化瑞香狼毒根乙酸乙酯萃取物，得到纯化合物后，用各种光谱技术确定化合物的化学结构，并利用浸玻片法测定化合物的杀螨活性，发现了这种植物中的狼毒色原酮能够明显地增长叶蟃的触杀率，推测黄酮类化合物是这种植物中的杀虫活性成分。从瑞香狼毒的提取物中分离得到了1个化合物，通过理化性质和波谱数据确定了它的化学结构，为狼毒色原酮。狼毒色原酮根乙酸乙酯中主成分为狼毒色原酮。 关键词：瑞香狼毒；狼毒色原酮；杀螨活性；抑菌活性 Stellera chameajasme ethyl acetate extract from the separation and purification of chemical constituents Name of student：Jin Kaiyan Name of tutor： Hua Baoguang，Cheng jun Abstract Stellera chameajasme on the chemical composition of ethyl acetate extract and acaricidal activity of research, search for lead compounds from plants miticide. Using a variety of chromatography for separation, purification Stellera chameajasme ethyl acetate extract, obtained after purification combined with various spectroscopic techniques to determine the chemical structure of compounds, and use dip slide method for the determination of the acaricidal activity of compounds and found that species of plants Chamechromone Leaf Man can significantly increase the contacting rate, suggesting that flavonoids are the plant of insecticidal active ingredients. Extract from Daphne chamaejasme isolated compounds 1, through the physical and chemical properties and spectral data to determine its chemical structure, as Chamechromone. Chamaechromone main components with acetic Chamechromone. Keywords Stellera chamaejasme，Chamechromone，acaricidal activity，antibacterial activity 目录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc262915066 目录 PAGEREF _Toc262915066 \h 2 HYPERLINK \l _Toc262915067 前言 PAGEREF _Toc262915067 \h 3 HYPERLINK \l _Toc262915068 1.瑞香狼毒生物活性的提取分离和纯化 PAGEREF _Toc262915068 \h 4 HYPERLINK \l _Toc262915069 1.1 实验材料 PAGEREF _Toc262915069 \h 4 HYPERLINK \l _Toc262915070 1.2 仪器与试剂 PAGEREF _Toc262915070 \h 4 HYPERLINK \l _Toc262915071 1.3 瑞香狼毒粗提物的系统溶剂提取方法 PAGEREF _Toc262915071 \h 4 HYPERLINK \l _Toc262915072 1.4 乙酸乙酯萃取部分活性物质的分离方法 PAGEREF _Toc262915072 \h 6 HYPERLINK \l _Toc262915073 1.5