磁刺激对星形胶质细胞迁移的影响及机制研究-康复医学与理疗学专业论文.docx

华 华 中 科 技 大 学 博 士 学 位 论 文 PAGE 5 PAGE 5 Effects of magnetic stimulation on astrocytes migration and underlying mechanism Doctor Candidator:Zhe Li Tutor:Prof. Xiaolin Huang Dapartment of Rehabilitation Medicine,Tongji Hospital,Tongji Medical College,Huazhong University Of Science and Technology ABSTRACT Part Ⅰ：Effects of magnetic stimulation with different intensities on astrocytes migration and underlying mechanism Exp Ⅰ：Effects of magnetic stimulation with different intensities on astrocytes migration 【Abstract】Objective To investigate the effects of magnetic stimulation with different intensities on astrocytes migration.Methods Twenty-four adult healthy Sprague-Dawley rats were selected and injected with 0.5μl of 1% ethidium bromide (EB) in PBS into the dorsal spinal cord funiculus of the left side at T10-11 level to make located spinal cord injury models and were randomly divided into four groups.The rats of four groups were exposed to pulsed magnetic stimulation(1Hz, 30 pulses) at the following intensities respectively: 0 Tesla(control group), 0.76 Tesla （Group 0.76T), 1.52 Tesla(Group 1.52T), 1.9 Tesla(Group 1.9T). On the day 14 after stimulation, the rats were sacrificed and the expressions of glial fibrillary acidic protein(GFAP), 5-Bromo-2′- deoxyuridine（Brdu），microtubule associated protein-2 (MAP-2) and the volume of the holes of injuried area of spinal cord were detected with immunohistochemistry and Western-Blot technique. Quantitative analysis of the expression of GFAP，Brdu and MAP-2 were performed with the image analysis system.Results With the increase of magnetic stimulation intensity, the volume of the holes of injuried area of spinal cord discrease at day 14. In the reduced area of the holes,the expressions of GFAP could be seen,while Brdu and MAP-2 could not be seen. Significant differences were revealed in the expressions of GFAP among the four groups，which were significantly higher in magnetic stimulation groups than that in control group (