《木耳菌糠木聚糖酶超声波提取工艺及部分酶学性质研究》》-毕业论文.docVIP

PAGE 题 目：木耳菌糠木聚糖酶超声波提取工艺及部分酶学性质 木耳菌糠木聚糖酶超声波提取工艺及酶学性质研究 摘要：木聚糖是半纤维素的主要成分之一，广泛存在于各种植物资源中。木聚糖酶是一类降解木聚糖分子的酶的总称，组成复杂，用途广泛。但目前木聚糖酶生产成本高，价格昂贵，限制了其广泛应用。而木耳菌糠中却含有一定量的木聚糖酶，且木耳菌糠廉价易得。本试验利用超声波法进行单因子试验和正交试验从木耳的菌糠中提取木聚糖酶。最佳工艺为：振幅为80%；总时间为20min；超声时间为10s；固液比为1：25，此时木聚糖酶活力最高为4.23（IU/g）。经过酶学性质分析，最佳pH为4.6；最适温度为45℃。 关键词： 木耳；菌糠；超声波；木聚糖酶；提取工艺 The Tecnology of Extracting Xylanase from Auricularia Auricula Residue with Ultrasonic Method and Enzymology Properties of Crude Xylanase Abstract: Xylan is a major component of hemicellulose, which widely exists in various plant resources. Xylanase is a kind of enzyme which decomposes xylan molecules, has complex composition and is used widely. But the production cost of xylanase is high and the price is expensive, which limits their wider application. While there is a certain amount of xylanase in the auricularia auricular residue, and the auricularia auricular residue is cheap and easy to get. In this study, the xylanase was extracted from the auricularia auricular residue through the single factor and orthogonal tests which was conducted with ultrasonic method. The results show that the optimum extracting technology was that amplitude was 80%; total time was 20min; ultrasonic time was 10s; solid-liquid ratio was 1:25, the highest xylanase activity under the optimum extracing technology was 4.23IU/g. The optimum pH and temperature of xylanase was 4.6 and 45 ℃. Keywords：Auricularia；Auricula residue ；Ultrasonic wave；Xylanase；Technology of Ectracting 目　　录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc261684209 1 前 言 PAGEREF _Toc261684209 \h 1 HYPERLINK \l _Toc261684210 2 材料与方法 PAGEREF _Toc261684210 \h 2 HYPERLINK \l _Toc261684211 2.1 材料 PAGEREF _Toc261684211 \h 2 HYPERLINK \l _Toc261684212 2.1.1原料及其处理方法 PAGEREF _Toc261684212 \h 2 HYPERLINK \l _Toc261684213 2.1.2主要仪器 PAGEREF _Toc261684213 \h 2 HYPERLINK \l _Toc261684214 2.1.3主要试剂及配制方法 PAGEREF _Toc261684214 \h 2 HYPERLINK \l _Toc261684215 2.2 方法 PAGEREF _Toc261684215 \h 4 HYPER