甘草中甘草酸提取工艺的优化论文04564.doc

绝密文件，核心资料，拒绝盗版， 绝密文件，核心资料，拒绝盗版， 支持正版，从我做起，一切是在为了方便大家！知识就是力量！ 支持正版，从我做起，一切是在为了方便大家！知识就是力量！ 甘草中甘草酸提取工艺的优化 内容摘要 甘草酸为甘草有效成分的重要组成部分。本论文以氨性醇作为提取溶剂，采用响应面试验方案，找出了其最佳提取条件：1.单因素处理时溶剂采用0.3%稀氨水加20%乙醇，处理时间为2.5h，处理温度为90℃。2.综合处理的最佳结果是当氨性醇溶液中稀氨水的浓度为0.338%，水浴处理温度为93.4℃，处理时间为2.665h时，所得的吸光度（abs）最佳abs=0.598401，对应的浓度是0.0467mg/ml，甘草酸的提取率为11.68%。 【关键词】甘草酸 氨性醇 响应面法 紫外可见分光光度计 Optimization of Extraction of glycyrrhizic acid in licorice Abstract Glycyrrhizic acid as part of an important component of the active ingredients of licorice. In this thesis, the ammoniacal alcohol as the extracting solvent, by response surface testing program, the optimum extraction conditions were identified: 1.A single factor treatment solvent with dilute ammonia solution of 0.3% plus 20% ethanol, the processing time of 2.5h, the processing temperature of 90 ℃.2. The best results of the integrated treatment of ammonia alcohol solution of dilute ammonia concentration of 0.338%, Water bath processing temperature was 93.4 ° C, processing time when 2.665h The resulting absorbance (abs) abs = 0.598401, corresponding to the concentration of 0.0467mg/ml ,Glycyrrhizic acid extraction rate of 11.68%. 【key words】:Glycyrrhizic acid Ammoniacal alcohol Response Surface Methodology UV-Vis spectrophotometer 目录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc295579609 一、引言 1 HYPERLINK \l _Toc295579610 （一）甘草及甘草酸简介 1 HYPERLINK \l _Toc295579611 （二）甘草酸提取工艺目前的状况 1 HYPERLINK \l _Toc295579612 （三）设计实验方案的依据 PAGEREF _Toc295579612 \h 2 HYPERLINK \l _Toc295579613 （四）设计实验方案 PAGEREF _Toc295579613 \h 2 HYPERLINK \l _Toc295579614 二、实验材料与方法 PAGEREF _Toc295579614 \h 2 HYPERLINK \l _Toc295579615 （一）实验仪器及试剂 PAGEREF _Toc295579615 \h 2 HYPERLINK \l _Toc295579610 （二）材料处理与试剂配制 2 HYPERLINK \l _Toc295579611 （三）实验方法与步骤 2 HYPERLINK \l _Toc295579617 三、结果与分析 PAGEREF _Toc295579617 \h 3 HYPERLINK \l _Toc295579618 （一）绘制标准曲线 3 （二） HYPERLINK \l _Toc295579619 单因素处理结果与分析 PAGEREF _Toc295579619 \h 3 HYPERLINK \