分光光度法测定金荞麦(-)表儿素含量的方法研究毕业论文.docx

分光光度法测定金荞麦(-)表儿茶素含量的方法研究姓 名： 学 号： 院 （系）：生物与制药工程学院 专 业：食品质量与安全 校内指导老师： 职称： 目录摘要、关键词…………………………………3前言……………………………………………3一、材料与方法………………………………3二、结果与分析………………………………4三、讨论与结论………………………………6参考文献………………………………………6致谢……………………………………………7分光光度法测定金荞麦(-)表儿茶素含量的方法研究 摘要：为建立香荚兰素-盐酸分光光度法对表儿茶素含量的测定方法，实验分别以乙醇和水作溶剂，在34.79～208.74ug范围内取样置50ml容量瓶测定，结果发现：①以乙醇作溶剂时，测定波长为508nm，1%香荚兰浓盐酸添加量应超过40ml(CV≤1.86%)，测定时间以40min后为宜(CV≤2.65%)，标准曲线线性关系(R＝0.9993)与精确度 (CV＝2.66%) 、准确度(p＞0.05)良好。②以水作溶剂时，测定波长为504nm，显色反应不稳定，标准曲线线性关系(R＝0.9881)也较一般。③采用乙醇作溶剂时，测得金荞麦块根中(-)表儿茶素类物质含量占干物质的2.22%(CV≤3.90%)，该法操作简便，灵敏度高，重现性好。关键词：(-)-表儿茶素；分光光度法；含量测定Study on the Determination of Epicatechin in Fagopyrum Dibotrys by SpectrophotometryAbstract: In order to establish a spectrophotometric determination of the vanillin-hydrochloric acid method to determine the content of epicatechin, the samples ranging from 34.79ug to 208.74ug contained in the 50ml volumetric flasks are determined with absolute alcohol and water as solvent respectively. The results show that: (1) When absolute alcohol is used as dissolvent, the detection wavelength is at 508 nm, the addition of the 1% vanillin-hydrochloric acid should exceed 40ml (CV≤1.86%), and the optimum detection period is 40min (CV≤2.65%). Meanwhile, the linear relation of the standard curve(R＝0.9993), the degree of precision (CV＝2.66%) and the accuracy of measurement(p＞0.05) show good. (2) When water is used as dissolvent, the detection wavelength is at 504 nm, but the chromogenic reaction is not stable and the linear relation of the standard curve is rather ordinary. (3) When absolute alcohol is adopted as dissolvent, the contents of epicatechin in fagopyrum dibotrys’ tuberous root account for 2.22% of the dry matter content (CV≤3.90%). Therefore, this is a research method with convenient operation, high sensibility and good reproducibility. Key words: epicatechin; spectrophotometry; determination前言金荞麦等一些药用植物根茎、果实中含有一类原花色素缩合性单宁混合物，其主要基本单元为(-)-表儿茶素[1]，表儿茶素具有显著的抗癌，抑制肿瘤细胞肺侵袭和转移，以及消炎抗菌等重要作用，是多种有效