shewanella oneidensis mr-1对硫化汞的生物利用性分析-bioavailability of shewanella oneidensis mr - 1 to mercuric sulfide.docx

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2018-06-03 发布于上海
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shewanella oneidensis mr-1对硫化汞的生物利用性分析-bioavailability of shewanella oneidensis mr - 1 to mercuric sulfide.docx

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shewanella oneidensis mr-1对硫化汞的生物利用性分析-bioavailability of shewanella oneidensis mr - 1 to mercuric sulfide

AbstractMercuryasaspecialheavymetalhaspaiedmoreandmoreattentionbypeoplebecauseofitseasilyglobalpollution.Inthenatur,mercuricsulfidewhichhasaverylowsolubilitywidelyexistsintheore，sedimentandsoil.Andanyformofmercurycantransformintomethylmercuryundertheactionofmicrobes.Sulfatereducingbacteriawhichisamercurymethylationmicroorganismwasmainlystudied,whiletheironreducingbacteriaShewanellaoneidensisMR-1hasbeenlittleresearched,speciallymercuricsulfideundertheactionofS.oneidensisMR-1hasbeenrarelyresearchedifitwillcausemercurypollutionintheenvironment.ThebiologicaldissolutionandmethylationofsolidHgSbyS.oneidensisMR-1andinfluencedfactorsunderlaboratoryconditionswasresearched.TheresearchprovideddirectevidencetobiologicalsolubilityandbiologicalmethylationonsolidHgSbyiron-reducingbacteriainthenaturalaquaticecosystem,andprovedthatthesolidmercuricsulfidewasalsooneofthemajorsourcesofenvironmentalmercurypollution.Themainresearchcontentsandresultswereasfollows：Throughthepureculturemicrobialexperiment,theironreducingbacteriaS.oneidensisMR-1hasacertaintoleranceofsulfideconcentration.ButS.oneidensisMR-1couldnotsurviveWhenthesulfideconcentrationwasexceeds3.4mg?L-1.S.oneidensisMR-1couldgrow,butitsgrowthwasinhibitedwhentheconcentrationofNa2Slessthan3mg?L-1,whichmainlyexpressedintheprolongedlagphasebuthadnosignificanteffectforthetimeoflogarithmicgrowthperiod.ThedifferentinfluencingfactorsofdissolutionofsolidHgSwithoutS.oneidensisMR-1wasresearched.TheresultsshowedthatthedissolutionofsolidHgSwouldslightlyincreaseunderdifferentinfluencingfactors,butthemaximumconcentrationwasnomorethan30μg?L-1.Inaddition,thesolubilityofHgSwasenhancedwiththeincreasingconcentrationofNa2S,andneutraloralkalineenvironmentwasmorebeneficialtodissolutionofHgSthanacidicandweaklyacidicconditions;thesolubilityofHgSwasenhancedwiththeincreasingconcentrationofhumicacid,butdifferentconcentrationsofcysteinehadnoeffecttothedissolvedquantityofmercuricsulfide.ThebiologicaldissolutionofsolidHgSbyS.oneidensisMR-1werestudiedunderlabor