毕业设计论文-核酶抗肿瘤和抗病毒作用的研究进展.doc

核酶抗肿瘤和抗病毒作用的研究进展 摘要 关键词： The research progress of ribozymes antitumor and antiviral effect Author: Wang Ruanna 　　　 Tutror: Li Abstract Ribozyme is a catalytic RNA having a biological activity. The ribozyme substrate is a RNA molecule, which catalyze the RNA cutting and splicing. Its function characteristics are: low cutting efficiency, easy to damage by RNase; catalytic types include RNA nucleotidyl acyl reaction, hydrolysis reaction (RNA restriction endonuclease reaction) and connection reaction (polymerase activity); also may have amino acid esterase, aminoacyl tRNA synthetase and peptidyl transferase activity, showed that ribozyme play plays an important role in Translation process and ribosome function. Currently found in the ribozyme includes: a class of intron, intron two class, RNaseP, hammerhead, hairpin, axehead ribozyme (HDV ribozyme). In recent years, confirmed, ribozyme exists widely in nature, its secondary structure has been studied clearly.Ribozyme is the catalyst that can be repeated action, compared with antisense RNA, ribozymes less medication dosage, toxicity is also smaller, and the role of the virus ribozyme targeting sequence is specific, and therefore more difficult to produce virus tolerance. Splicing ribozyme use of highly specific diseases treatment corresponding application. Ribozyme technology as a promising tool in gene therapy, although 20 years have had great development, but there are many problems remain to be solved, such as the ribozyme in vitro screening of high activity, strengthen the research on lenticular, axe ribozymes in vivo expression system, the establishment of efficient, enhance ribozyme within cells. The cutting efficiency, seek more effective vectors etc. To solve these problems, will undoubtedly make the ribozyme to further shorten the time in clinical application. Although the ribozyme antiviral, antitumor effect