片仔癀肝宝对大鼠解酒效果及慢性酒精肝治疗的研究.doc

复方片仔癀肝宝对酒精诱导的慢性肝损伤氧化应激的保护作用研究陈达鑫1,2 林珊1,2 赵锦燕1,2 曾建伟1,2 洪振丰1,21福建中医药大学中西医结合研究院 福州350122；2福建省中西医结合老年性疾病重点实验室 福州350122 摘要：目的探讨复方片仔癀肝宝对酒精性肝病leoholie Liver Disease，ALD模型大鼠氧化应激的保护作用。方法60只SPF级SD大鼠，随机分为对照组、模型组、阳性药对照组肝宝低、中、高剂量组每组10只。除正常对照组外其余各组用酒精联合高脂饲料喂养2周，造ALD模型，连续给药4周后取材。全自动生化仪检测血清中谷丙转氨酶（ALT）谷草转氨酶（AST）碱性磷酸酶（ALP）乳酸脱氢酶（LDH）的含量变化；检测肝组织中超氧化物歧化酶（SOD）丙二醛（MDA）和谷胱甘肽过氧化物酶（GSH-Px）的表达；HE染色，观察肝组织病理变化。结果模型组血清中ALT、AST、ALP、LDH的活性较均有明显的上升，且肝组织中MDA水平明显上升，SODGSH-Px水平明显下降（P＜0.01）。与模型组相比，肝宝各组ALT、AST、ALP、LDH的活性及MDA水平明显降低，而SOD、GSH-Px水平明显上升（P＜0.05）。HE染色结果显示复方片仔癀肝宝不同剂量组对肝组织脂肪变性、肝内脂类聚集有明显的改善作用，并且存在剂量效应关系。结论复方片仔癀肝宝能显著减轻酒精和高脂诱导的肝损伤，其机制可能与提高肝组织的抗氧化能力有关。 关键词：酒精性肝损伤；片仔癀肝宝；氧化应激 Complex Prescription Pien Tze Huang Ganbao Protects Against Alcohol-induced Chronic Liver Injury Caused by Oxidative Stress CHEN Da-xin1,2, LIN Shan1,2, ZHAO Jin-yan1,2, ZENG Jian-wei1,2, HONG Zhen-feng1,2* (1Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou350122; 2Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou350122) Abstract: Objective:?To determine the protective effect of complex prescription Pien Tze Huang Ganbao (GB) in alcoholic liver disease (ALD) using a rat model of oxidative stress.?Methods:?60 SPF SD rats were randomly divided into control group, model group, positive control group, GB low, medium and high dose groups (n=10 for each group).?All groups except control group were fed an alcohol and high fat diet for two weeks, as a model for ALD, and rats were sacrificed following 4 weeks of feeding.?The level of expression of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were detected with automatic biochemical analyzer, as well as the expression of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in liver tissue. Using HE staining, pathological changes were observed in