幽门螺杆菌感染和环氧合酶.doc

幽门螺杆菌感染和环氧合酶-2表达在分泌性中耳炎患者相关性的研究 作者单位 通讯作者：刘强，Email:lqiange001@ 摘要 目的：探索环氧合酶-2和幽门螺杆菌感染在分泌性中耳炎中的相关性。方法：54例中耳手术的慢性分泌性中耳炎患者，CLO test方法检测幽门螺杆菌感染，同时例患者和27例正常人应用14C urea breath test (14C UBT) 方法检测患者中耳和胃幽门螺杆菌感染。法检测患者中耳组织环氧合酶-2的表达。结果：CLO test检测阳性的患者共37例。患者中14C UBT 例阳性（），而27例正常人中有7例阳性（25.9%）。法检测例患者中耳组织环氧合酶-2表达阳性，和幽门螺杆菌感染具有显著正相关。结论：分泌性中耳炎患者中耳组织存在幽门螺杆菌感染和环氧合酶-2表达，且二者存在正相关。患者中耳幽门螺杆菌感染的来源可能是胃。 关键词：环氧合酶-2Helicobacter pylori infection and cyclooxygenase -2 expression in the secretory otitis media in patients with Correlation Abstract Objective: To explore the COX -2 and Helicobacter pylori infection in secretory otitis media patients. Methods: A total of 54 cases of chronic secretory otitis media patients with middle ear surgery were selected. CLO test was performed to detect H. pylori infection, while 54 patients and 27 normal controls were applied for 14C urea breath test (14C UBT) in stomach of patients with secretory otitis media. Immunohistochemistry was performed to detect the COX -2 expression in middle ear of patients. Results: CLO test-positive patients were detected in 37 cases. 46 patients with 14C UBT 32 were positive (84.2%) among 54 patients, while 7 cases were positive among 27 cases of normal control (25.9%). 43 cases of patients with middle ear organizations were COX -2 expression positive detected by immunohistochemistry. Conclusion: There are higher infection rate of Helicobacter pylori in secretory otitis media patients compared to normal control both in middle ear and stomach. The source of Helicobacter pylori infection in patients with middle ear may be the stomach. Key words: Helicobacter pylori; Cyclooxygenase -2; secretory otitis media 分泌性中耳炎是以传导性聋及鼓室积液为主要特征的中耳非化脓性炎性疾病。冬春季多发，是小儿和成人常见的听力下降原因之一。其中主要致病菌为流感嗜血杆菌和肺炎链球菌、幽门螺杆菌近年来的研究发现中耳积液中细菌培养阳性者约为1/3～1/2，其中主要致病菌为流感嗜血杆菌和肺炎链球菌、幽门螺杆菌。笔者通过近年来的临床观察发现在分泌性中耳炎患者幽门螺杆菌感染与环氧合酶-2的表达有密切的相关性。现将结果报告如下： 对象与方法 观察对象 ①慢性分泌性中耳炎患者：本研究所观察例均为在我院门诊或住院治疗的分泌性中耳炎患者。其中男例，女4例；年龄16～62岁平均(36.02±2.93)岁；病程1至个月，平均(.7±0.7)个月发病部位：单耳例，双耳4。①临床症状：具有听