油菜柠檬酸合酶基因的克隆及在逆境下的表达-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2009, 35(1): 33−40 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.00033 油菜柠檬酸合酶基因的克隆及在逆境下的表达 童 晋 詹高淼 王新发 刘贵华 华 玮 王汉中* / , 430062 : , , cDNA , , cDNA , 1 659 bp, ORF 476 , (96.0%), , , PCR , , ; IAA 6-BA , , , ABA IAA ; ; GA3 PCR , PCR : ; ; ; Cloning of Citrate Synthase Gene in Rapeseed (Brassica napus L.) and Its Expression under Stresses * TONG Jin, ZHAN Gao-Miao, WANG Xin-Fa, LIU Gui-Hua, HUA Wei, and WANG Han-Zhong Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China Abstract: As a key enzyme of some metabolisms, citrate synthase shows a sign for metabolism. In order to investigate the func- tion of citrate synthase gene, the cDNA encoding citrate synthase was cloned from rapeseed leaf by RT-PCR. It was 1 659 bp long and encoded a protein with 476 amino acids. The deduced protein sequence had a mitochondrial targeting signal in N-terminal, which was very similar to the citrate synthase in Arabidopsis thaliana (96.0%). Alignment analysis showed that citrate synthase gene had high homology in plants. Under different stresses, we tested the expression of citrate synthase gene in rapeseed leaf by using semi-quantitative PCR. The expression of citrate synthase gene had no obvious change in stresses of salt, dark, high illumi- nation, while was increased at diffe