细胞过继免疫治疗肿瘤的临床研究进展 课件.ppt

* * * * * Whole blood (70 ml) was collected in BD Vacutainer blood collection tubes with sodium heparin and directly centrifuged to isolate PBMCs. PBMCs were stimulated with 5-mM zoledronic acid (Novartis, Basel, Switzerland) in AlyS203-gd medium (Cell Science and Technology Institute, Sendai, Japan) containing 1000 IU ml1 human recombinant IL-2 and 10% autologous serum at the beginning of the culture. * * Schemes for adoptive transfer of autologous, vaccine-primed, in vitro–expanded T cells. Patients are primed with tumor vaccine followed by lymphocyte harvest. Autologous T cells are harvested from peripheral blood (i) or draining lymph nodes (ii), undergo polyclonal in vitro activation and expansion, and are reinfused after lymphodepleting chemotherapy. Antigen-specific immune function is measured after the administration of booster vaccines. (iii) TILs can be isolated from resected surgical specimens and expanded in vitro for adoptive transfer after lymphodepleting chemotherapy. Most adoptive transfer therapy approaches using TILs have involved the use of IL-2 infusion following T cell transfer in order to select tumor-specific T cells. 转TCR基因T细胞治疗转移性黑色素瘤 Adoptive Cell Therapy Genetic Modification to Redirect Effector Cell Specificity Steven A. Rosenberg, Cancer J 2010;16: 336–341 转CAR基因T细胞过继免疫治疗B细胞 淋巴瘤 Eradication of B-lineage cells and regression of lymphoma in a patient treated with autologous T cells genetically engineered to recognize CD19, Steven A. Rosenberg, Blood, 2010,116: 4099-4102 PR 32周 扶正祛邪，辨证施治，个体化治疗 免疫监视理论： RAG2?/?小鼠诱发的强免疫原性肿瘤在野生型 小鼠不长；器官移植→免疫抑制→癌 肿瘤免疫编辑 （Cancer Immunoediting）理论： 免疫清除、对抗和逃逸。动态失衡、平衡过程。 免疫系统既可识别和杀伤肿瘤，又能促癌。 增强免疫，对抗肿瘤免疫逃逸，与常规疗法联 合将会大幅度提高疗效。 结束语 机遇：细胞免疫疗法与三大常规疗法互补，细胞 免疫疗法仅仅开始；与国外差距小，甚至 可走到世界前列。 挑战：疗效有限，改进方法潜力大。 健康科学发展，造福于患者！ 谢谢！ * * * * * * * * * * * * Figure 2 Intracellular cytokine pr