mtor通路抑制剂对卵丘扩展与体外受精的影响.pdf

《中国医学前沿杂志 （电子版）》2016 年第8 卷第7 期 ● ●  45  论著  mTOR 通路抑制剂对卵丘扩展与体外 受精的影响 公绪红，时兰英，苏友强 （南京医科大学　生殖医学国家重点实验室，南京　2 11166 ） 【摘要 】目的 研究哺乳动物雷帕霉素靶蛋白 （mammalian target of rapamycin ，mTOR ）通路抑制剂对 卵丘及卵母细胞发育潜能的影响。方法 收集小鼠较大有腔卵泡中卵丘- 卵母细胞复合体 （cumulus- oocyte complexes，COCs ）作为体外培养的研究对象，在培养液中加入不同浓度的mTOR 通路抑制剂 （雷帕霉素、Torin 1），分析mTOR 通路抑制剂对卵丘及卵母细胞发育潜能的影响。结果 雷帕霉素、 Torin 1 可以有效抑制COCs 中mTOR 信号通路，Torin 1 有明显影响卵丘细胞扩展的作用，使卵母细胞 受精及着床前胚胎发育潜能下降。结论 mTOR 信号通路抑制剂有明显降低卵母细胞质量的效应，是 维持COCs 活性及促进卵丘和卵母细胞的发育潜能所必需。 【关键词 】哺乳动物雷帕霉素靶蛋白；雷帕霉素；Torin 1 ；卵母细胞；卵丘细胞 Study on mTOR inhibitors to cumulus expansion and in vitro fertilization GONG Xu-hong, SHI Lan-ying, SU You-qiang (State Key Laboratory of Reproductive Medicine, Nanj ing Medical University, Nanj ing 2 11166, China) Corresponding author: SU You-qiang, E-mail: youqiang.su@nj mu.edu.cn Abstract Objective 【 】 　To investigate the influence of mammalian target of rapamycin (mTOR) inhibitors on cumulus cells and oocyte. Method　Collected cumulus-oocyte complexes (COCs) of large follicles in mice ovaries for in vitro culture, by added different concentrations of mTOR inhibitors in culture medium, analyzed its influence on cumulus cells and oocyte. Result　Rapamycin, Torin 1 could effectively inhibit the transmission of mTOR signaling pathway in COCs, and the ability of COCs cultured with Torin 1 to undergo cumulus expansion and the competence of oocyte for fertilization and supporting preimplantation development were dramatically compromised. Conclusion　mTOR inhibitors can significantly decrease oocyte quality, and it indicates that mTOR signal pathway is necessary to maintain COCs activity and its developmental competence. Key words 【 】mTOR; Rapamycin; Torin 1; Oocyte; Cumulus cells 哺乳动物雷帕霉素靶蛋白 （mammalian