荧光染料光谱列表.doc

荧光染料光谱列表染料名称 Excitation(nm) Emission(nm) 分子量 备注信息 Reactive and conjugated probes Hydroxycoumarin 325 386 331 Succinimidyl ester Aminocoumarin 350 445 330 Succinimidyl ester Methoxycoumarin 360 410 317 Succinimidyl ester Cascade Blue (375);401 423 596 Hydrazide Pacific Blue 403 455 406 Maleimide Pacific Orange 403 551 ? ? Lucifer yellow 425 528 ? ? NBD 466 539 294 NBD-X R-Phycoerythrin (PE) 480;565 578 240 k ? PE-Cy5 conjugates 480;565;650 670 ? aka Cychrome, R670, Tri-Color, Quantum Red PE-Cy7 conjugates 480;565;743 767 ? ? Red 613 480;565 613 ? PE-Texas Red PerCP 490 675 ? Peridinin chlorphyll protein TruRed 490,675 695 ? PerCP-Cy5.5 conjugate FluorX 494 520 587 GE Healthcare Fluorescein 495 519 389 FITC; pH sensitive BODIPY-FL 503 512 ? ? TRITC 547 572 444 TRITC X-Rhodamine 570 576 548 XRITC Lissamine Rhodamine B 570 590 ? ? Texas Red 589 615 625 Sulfonyl chloride Allophycocyanin (APC) 650 660 104 k ? APC-Cy7 conjugates 650;755 767 ? PharRed Alexa Fluor系列荧光染料 Alexa Fluor 350 343 442 410 ? Alexa Fluor 405 401 421 1028 ? Alexa Fluor 430 434 540 702 ? Alexa Fluor 488 499 519 643 QY 0.92 Alexa Fluor 500 503 525 700 ? Alexa Fluor 514 517 542 714 ? Alexa Fluor 532 530 555 724 QY 0.61 Alexa Fluor 546 561 572 1079 QY 0.79 Alexa Fluor 555 553 568 1250 QY 0.1 Alexa Fluor 568 579 603 792 QY 0.69 Alexa Fluor 594 591 618 820 QY 0.66 Alexa Fluor 610 610 629 1285 ? Alexa Fluor 633 632 648 1200 ? Alexa Fluor 647 652 668 1300 QY 0.33 Alexa Fluor 660 663 691 1100 ? Alexa Fluor 680 680 702 1150 ? Alexa Fluor 700 696 719 1400 ? Alexa Fluor 750 752 776 1300 ? Alexa Fluor 790 782 804 1750 ? Cy系列荧光染料 Cy2 489 506 714 QY 0.12 Cy3 (512);550 570;(615) 767 QY 0.15 Cy3B 558 572;(620) 658 QY 0.67 Cy3.5 581 594;(640) 1102 QY 0.15 Cy5 (625);650 670 792 QY 0.28 Cy5.5 675 694 1128 QY 0.23 Cy7 743 767 818 QY 0.28 核酸相关探针染料 Hoechst 33342 343 483 616 AT-selective DAPI 345 455 ? AT-selective Hoechst 33258 345 478 624 AT-selective SYTOX Blue 4