四种添加物对铁皮石斛原球茎生长及多糖含量的影响.pdf

2014 22(3) 257 ~ 262 1,2 1* 2 , , ()1/2MS 4 4 15.0 g L1 60 d (146.1%) 20.0 g L1 40 d (50.4%) doi: 10.3969/j.issn2014.03.007 - BAO Ying-hua1,2, PAN Chao-mei1*, BAI Yin2 (1. , Guangzhou 510006, China; 2. , Shaoguan 512005, China) In order to understand the function of additive in medium of , four additives, including coconut meat, sugarcane bagasse, banana peel and wheat bran, were supplied in 1/2MS medium, respectively. The effects of additives with different concentrates and culture time on growth and polysaccharide content of PLBs were studied. The results showed that the PLBs proliferation, differentiation and the polysaccharide content were infl uenced by four additives. The proliferation rate of PBLs reached up to 146.1% supplemented with 15.0 g L1 sugarcane bagasse and cultured for 60 d. The polysaccharide content of PLBs was increased for 50.4% supplemented with 20.0 g L1 sugarcane bagasse and cultured for 40 days. So it was suggested that sugarcane bagasse was suitable additive for PLBs owing to promote the growth of PLBs and reduce cost of production. ; Additive; PLB; Polysaccharide (Kimura et Migo) (Orchidaceae) [1 2] 2014 01 142014 02 04 (2011B060400004) E-mail: byhm@163.com * Corresponding author. E-mail: pancm@ 258 22 [3 5] (2010 ) (%) = ( )/ [6] × 100%(2010 )