高压液相色谱法同时测定食品中抗坏血酸与异抗坏血酸的含量.pdfVIP

4 1 Vol. 4 No. 1 2013 2 Journal of Food Safety and Quality Feb. , 2013 1 1,2* 张 清 , 李 刚 (1. , 130118; 2. , 130022) 摘 要: 目的 方法 −0.1% (9010, v/v), Venusil HILIC (250 mm×4.6 mm, 5 μm, 100 Å) 结果 96.61%, 96.53%, 0.35 μg/mL 0.42 μg/mL结论 , 关键词: ; ; ; Venusil HILIC Simultaneous determination of ascorbic acid and isoascorbic acid in food by high pressure liquid chromatography 1 1,2* ZHANG Qing , LI Gang (1. College of Food Science and Technology, Jilin Agricultural University, Changchun 130118, China; 2. Jilin Product Quality Supervision and Inspection Research Institute, Changchun 130022, China) ABSTRACT: Objective To establish a high pressure liquid chromatography (HPLC) method for simultaneous determination of the contents of ascorbic acid and isoascorbic acid in food. Methods Ascorbic acid and isoascorbic acid were separated using a Venusil HILIC column (250 mm×4.6 mm, 5 μm, 100 Å), taking acetonitrile-0.1% phosphoric acid solution (9010, v/v) as the mobile phase and extractant. Results The average recoveries of ascorbic acid and isoascorbic acid were 96.61% and 96.53%. And their minimum detectable concentration wa