不同麻醉方法建立大鼠肺鳞癌模型的研究-第三军医大学学报.docVIP

大鼠肺鳞癌癌变过程中细胞周期调控蛋白的动态表达( 刘文斌，刘晋袆，曹佳 (第三军医大学军事预防医学院军事毒理学教研室， 重庆 400038) 蛋白p16p27和p53水平动态变化趋势、相互关系及其在癌变过程中的作用。　支气管灌注3－甲基胆蒽(MCA)及二乙基亚硝胺(DEN)碘油溶液诱发Wistar大鼠肺鳞癌。采用免疫组化SP法检测p16, p27和p53蛋白在大鼠肺鳞癌癌变各阶段的表达水平，采用Western blot检测鳞癌、癌旁和正常组织中各蛋白表达水平。　共获取癌变各阶段标本154例，其中增生25例，27例，不典型增生37例，原位癌30例，浸润癌25例。p16和p27蛋白表达的阳性率随癌变进展而降低p53蛋白表达的阳性率随癌变进展而升高。p16与p27蛋白表达呈正相关(P 0.01)，p16和p27蛋白表达与p53蛋白表达呈负相关(P 0.0)。鳞癌组织中p16与p27蛋白低表达，p53蛋白高表达，与正常组织或癌旁组织有显著性差异(P 0.01)。　癌变p16和p27 p53蛋白大鼠肺鳞癌。 肺肿瘤；癌变；p16；p27；p53Dynamic Expression of Cell Cycle Regulation Related Protein during Carcinogenesis of Lung Squamous Cell Carcinoma in Rats LIU Wen-bin, LIU Jin-yi, ZHOU Zi-yuan, ZHOU Yan-hong, AO Lin, CUI Zhi-hong, CAO Jia (Department of Hygienic Toxicology, Preventive Medicine College, Third Military Medical University, Chongqing 400038，China) Abstract Objective To investigate dynamic expression and correlation of cell cycle regulation related protein p16, p27 and p53 during carcinogenesis of chemically-induced lung squamous cell carcinoma in Wistar rats. Methods Squamous cell carcinoma of lung was induced with 3-methylcholanthrene (MCA) and diethyinitrosamine (DEN) in iodized oil by left intra-bronchial instillation in 80 Wistar rats. SP method was used to examine the expressions of p16, p27 and p53 protein during rat lung carcinogenesis. Normal tissue, tumor tissue and corresponding adjacent noncancerous tissues were examined by western blot for the protein expressions of p16, p27 and p53. Results A total of 154 specimens of various pathological phase during carcinogenesis were obtained: 25 hyperplasia, 27 squamous metaplasia, 37 dysplasia, 30 carcinoma in situ and 25 infiltrating carcinoma. The positive rates of p16 and p27 proteins decreased and the positive rate of p53 protein increased obviously when the carcinoma developed. The expression of p16 was positively correlated with p27 (P 0.01). The expression of p53 was negatively correlated with p16 and p27 (P 0.05). The loss of p16 and p27 expression, p53