右美托咪定对大鼠肺移植后肺缺血再灌注损伤的影响宋兆孟陈旭.docVIP

右美托咪定对大鼠肺移植后肺缺血再灌注损伤的影响 宋兆孟陈旭蒋琦亮徐美英 基金项目：；（YZ13-28）作者单位：200062上海交通大学附属儿童医院（宋兆孟）；上海交通大学附属胸科医院（陈旭蒋琦亮徐美英） 通信作者：徐美英，xumeiyingxk@163.com 【摘要】 目的 评价右美托咪定DEX）对大鼠肺移植后肺缺血再灌注损伤（LIRI）的影响。方法 无特殊病原体（SPF）级雄性Sprague-Dawley大鼠30只，体重250～350 g，2～3月龄，采用随机数字表法，将分假手术组、肺移植组和DEX。假手术组开胸游离左肺门，未行肺移植；肺移植组左肺移植后开放再灌注2 h；DEX于移植肺再灌注前30 min腹腔注射DEX 25 ugkg。再灌注2 h后取移植肺组织，测定IL-1β、IL-10、TNF-α髓过氧化物酶（MPO）丙二醛（MDA）；光镜下观察肺组织病理学改变。结果 肺移植组和DEX组IL-1β、IL-10、TNF-αMDA、MPO假手术组P值均0.05）DEX组IL-1β、TNF-αMDA、MPO肺移植组P值均0.05），IL-10肺移植组P0.05）光镜（×200）下，与肺移植组比较，DEX组肺组织病理学损伤明显减轻，可见少量炎性细胞浸润，肺泡结构较清晰。结论 DEX可减轻大鼠肺移植后的肺缺血再灌注损伤，其机制可能与抑制炎反应和氧化应激有关。 【关键词】右美托咪定；肺移植；缺血再灌注损伤 ffect of dexmedetomidine in lung ischemia-reperfusion in rats after lung transplantation *SONG Zhaomeng，CHEN Xun，JIANG Qiliang，XU Meiying. * Shanghai Children Hospital,Shanghai Jiaotong University,Shanghai 200062,China Corresponding author: Xu Meiying. E-mail : xumeiyingxk@163.com 【Abstract】 Objective To evaluate the effect of dexmedetomidine lung ischemia-reperfusion injury(LIRI) after lung transplantation in rats. Methods Thirty pathogen-free male Sprague-Dawley rats，weighing 250-350 g，aged 2-3 monthswere divided into 3 groups according to random number table(n=10 each)：sham operation group (group S)；lung transplantation group(group L)；dexmedetomidine group (group D)． The donor lungs were removed from live donor rats and placed in low potassium dextran solution at 4℃．rthotopic left lung transplantation, the transplanted lung was reperfused for 2h．In group D, dexmedetomidine 25 ugkg was injected intraperitoneally 30 min before reperfusion． determination of IL-1β, IL-10, TNF-α, malondialdehyde(MDA) and myeloperoxidase(MPO) levels． Results Compared with group S，IL-1βIL-10, TNF-α, MDA and MPO were significantly increased in group L and group D(all p0.05)．Compared with group L，IL-1βTNF-α, MDA and MPO were significantly decreased， IL-10 was significantly increased in group D(all p0.05). It was found that pathological changes of lungs were significantly atte