三维多孔复合人工骨制备及性能检测.doc

三维多孔复合人工骨制备及性能检测 　 作者：宋世锋，彭磊，王臻，常琪，孙铮，滕勇，李智，孟国林，崔玉明 【摘要】 ［目的］研制新型三维多孔复合人工骨材料并评价其相关性能。［方法］将海洋贝壳牡蛎粉、消旋聚乳酸按一定比例复合，采用热致相分离法 制备多孔复合人工骨（CAB）材料，检测其孔隙率、孔径、生物力学强度；并将CAB和纯PLLA薄片浸泡于37℃平衡液中，观测不同时间点CAB和纯PLLA体外降解变化参数,对其结果进行统计学比较。［结果］研制的CAB材料平均孔隙率为85. 1％；电镜下孔径测量大小为100～ 300 μm，孔隙之间的连通较好，孔隙形态、取向规则有序；压缩强度为2. 12 MPa；在观测周期内，随着浸泡时间的延长，CAB和纯PLLA的质量损失率、浸泡液pH值呈现出规律性变化，两组各时期的各项指标比较具有显著性差异（P﹤0. 05）。 ［结论］ 该法制得的CAB材料的孔隙率、孔径、生物力学强度、体外降解性能表明能满足骨替代材料的要求。 【关键词】 骨替代材料; 牡蛎粉; 聚乳酸; 人工骨; 热致相分离法 Abstract：［Objective］To prepare a new porous composite artificial bone substitute and evaluate its properties.［Method］Thermally induced phase separation was adopted to prepare the artificial bone made from oyster shell powder and PLLA which were proportionally mixed and its properties as porosity rate,pore size and mechanical strength were assessed.Meanwhile the related variation parameters were examined every 2 weeks in a course of 14 weeks after the slices of CAB and pure PLLA were immersed in NS of 37℃ in vitro ，the results of which were compared in statistics.［Result］The average porosity rate of artifical bone with TIPS method was 85.1% and pore sizes ranged 100-300 μm under the SEM,with better pore connectivity and regulation shape.The average compressive strength was 2.12 MPa.As the immersion prolonged,the regular variation was observed about the mass loss of CAB and the pH alteration of solution,there was statistically difference in the indexes between the two groups （P﹤0.05）.［Conclusion］The porosity rate,pore size,mechanical strength and　degradation performance in vitro of the artifical bone made by TIPS method can satisfy the requirement of bone substitute . Key words：bone substitute; oyster shell power; polyLlactic acid; artifical bone; TIPS 骨缺损修复一直是困扰着矫形外科和骨科医生的一个难题，每年都有大量的病人因各种原因导致骨缺损难以修复，人工骨移植材料在最近几十年得以迅速发展，但或多或少都存在价格昂贵，来源紧张，其综合性能难以令人满意。作者所采用的牡蛎壳粉为海南三亚海域近江贝壳粉末，其主要成分为文石型碳酸钙，是一种理想的生物源性成骨材料。碳酸钙为主体的生物材料因其本身的Ca2+、CO2-3存在于正常人体液中，其具有更为良好的生物相容性，与人体骨成长过程的类似性，成为近几年来骨修复体研究的新热点。有机高分子材料消旋聚乳酸（PLLA）具有降解快且完全、成