Survivin及C-myc在肝门部胆管癌组织中表达及其相关性探究.doc

Survivin及C-myc在肝门部胆管癌组织中表达及其相关性探究 　 作者：韩兴华　智绪亭　刘清波　孙汉臻　石晓岩 【摘要】 　　目的：探讨Survivin和C-myc在肝门部胆管癌中的表达、临床意义及相互关系。方法: 用免疫组织化学SP法检测45例肝门部胆管癌及20例非癌胆管组织中Survivin和C-myc的表达。结果: 45例肝门部胆管癌组织中Survivin和C-myc的表达率分别为68.9%（31/45）和62.2%（28/45），非癌胆管组织中Survivin和C-myc的表达率分别为0和15%（3/20），差异有统计学意义（Plt;0.05）。肝门部胆管癌组织中Survivin的表达与淋巴结转移有关（Plt;0.05），C-myc的表达与肿瘤分化程度和淋巴结转移有关（Plt;0.05）,它们的表达与患者的性别、年龄，肿瘤大小及Bismuth分型无关（Pgt;0.05）。Survivin、C-myc的表达呈正相关关系（Plt;0.05）。 结论: Survivin、C-myc在肝门部胆管癌中的表达呈正相关关系，共同在肝门部胆管癌的发生、发展、转移中起一定作用，Survivin有望成为肝门部胆管癌治疗的新靶点。 【关键词】 胆管肿瘤　Survivin　基因，myc 　　 Expression and correlation of Survivin and C-myc in 【ABSTRACT】 Objective: To investigate the expression of Survivin and C-myc with the clinical significance of hilar cholangiocarcinoma and the correlation between the expressions of Survivin and C-myc. Methods: The expressions of Survivin and C-myc in 45 cases of hilar cholangiocarcinoma and in 20 cases of non-carcinoma bile duct tissues were examined by immunohistochemistry. Results: In 45 cases of hilar cholangiocarcinoma, the positive rates of Survivin and C-myc were 68.9%（31/45）and 62.2%（28/45）, the positive rates of Survivin and C-myc in 20 cases of non-carcinoma bile duct tissues were 0 and 15%（3/20）respectively, the difference was significant (Plt;0.05). The expression of Survivin in hilar cholangiocarcinoma was related with lymph node metastasis (Plt;0.05), the expression of C-myc was related with differentiated degree and lymph node metastasis(Plt;0.05). The expressions of Survivin and C-myc had no association with gender（Pgt;0.05）, age（Pgt;0.05） and tumor size（Pgt;0.05）. The correlation between the expressions of Survivin and C-myc was significant (Plt;0.05). Conclusions: The expression of Survivin was significantly related with expression of C-myc in hilar cholangiocarcinoma. 【KEY WORDS】Bile duct neoplasms·Survivin·Genes，myc·Immunohistochemistry 　　本研究应用免疫组化SP法检测肝门部胆管癌中Survivin与C-myc的表达，探讨二者在肝门部胆管癌的形成、发展和转移中的作用及其相互关系。 1　材料和方法 1.1　标本来源　山东大学齐鲁医院1999～2005年行根治性切除、病