探讨别嘌呤醇抗氧化作用机制在改善心衰大鼠心功能中的作用.docVIP

探讨别嘌呤醇抗氧化作用机制在改善心衰大鼠心功能中的作用 王真真，刘地川，蔡 敏，吴健 （400010 重庆，重庆医科大学附属第二医院心血管内科） [摘要]目的 。方法 雄性SD大鼠35 只200±20）g，（AMI+ALLO，（AMI+NS，（operated +NS，NS）灌胃。8周后，检测大鼠心功能血清脑钠尿肽（Brain Natriuretic PeptideBNP）含量黄嘌呤氧化酶（Xanthine OxidaseXO）蛋白表达丙二醛（MalondialdehydeMDA）含量线粒体呼吸链酶Ⅰ-Ⅳ的活性心肌组织形态以及心肌诱导型一氧化氮合酶（induce-nitri-coxide synthaseiNOS）的表达量。结果与组相比，大鼠心功能、线粒体呼吸链酶ⅠⅢ、Ⅳ活性明显（P0.05，血清BNP含量、XO蛋白表达、MDA水平明显升高（P0.05HE染色显示心肌组织明显水肿（P0.05，iNOS 表达升高（P0.05；（EF：87.10%与（EF：68.18%）相比大鼠心功能明显改善（P0.05，BNP含量、XO蛋白表达、MDA水平均下降（P0.05，大鼠线粒体呼吸链酶ⅠⅣ活性增加（P0.05，HE染色显示心肌组织水肿减轻（P0.05iNOS表达受抑制（P0.05。结论；ALLO可以改善大鼠的心功能，其机制可能与。 [关键词] 别嘌呤醇；活性氧；心力衰竭；心肌能量代谢； Iantioxidant mechanism on improving cardiac function of Chronic Heart Failure Rats WANG Zhenzhen，LIU Dichuan，CAI Min，WU Jian （Department of Cardiology， Second Affiliated Hospital of Chongqing Medical University，Chongqing 400010，China） [Abstract] Objective To determine the protective effect of ALLO (allopurinolALLO) on cardiac function in rat with chronic heart failure and its antioxidant mechanism in improving cardiac function. Methods Thirty-five of 6-weeks-old male SD rats average weighted (200 ± 20)g, randomly divided into the ALLO treatment group (n=12),control group (n=13) and sham-operated group (n=10).SD rats in ALLO treatment group and the control group were established of acute myocardial infarction (acute myocardium infarction, AMI) models by ligation of the left anterior descending coronary artery, and SD rats of sham-operated group underwent the same procedure, including anesthesia and open-chest surgery, but the LAD was not ligated. Two days after the surgery,ALLO treatment group animals underwent gavage administration by Allo(50mg/kg?d),while the gavage administration in control group and sham-operated group animals were only by the saline .Eight weeks later,the cardiac function of SD rats,levels of BNP ,expression of xanthine oxidase(XO), Malondialdehyde (MDA), the activity of mitochondrial complexⅠ-Ⅳ, morphological changes in myocardial tissue and the expression o