后基因组时代下作物的SNP分型方法.PDFVIP

分子植物育种，2010 年，第8 卷，第1 期，第125-133 页 Molecular Plant Breeding, 2010, Vol.8, No.1, 125-133 评述与进展 Review Progress 后基因组时代下作物的SNP 分型方法 1 2 3 4 3 1* 赵琼一 李信 周德贵 何飞 周少川 罗达 1 中国科学院上海植物生理生态研究所, 植物分子遗传国家重点实验室, 上海, 200032; 2 上海交通大学, 生命科学技术学院, 上海, 200030; 3 广 东省农业科学院水稻研究所, 广州, 510640; 4 上海生物芯片有限公司, 生物芯片上海国家工程研究中心, 上海, 201203 * 通讯作者, dluo@ 摘 要 单核苷酸多态性(single nucleotide polymorphisms, SNPs)是生物体最普遍的一种多态差异，在植物 功能基因组研究和作物遗传改良方面有着广泛的应用。利用全基因组水平SNP 标记谱进行遗传变异的研 究、群体结构分析、关联性分析、作物分子设计育种，以及对大规模SNP 数据进行验证、评估等，都迫切需要 发展和利用各种不同的SNP 分型手段实现。本文综述了目前常用的一些SNP 分型方法，简要介绍了检测原 理及操作流程，并对后基因组时代下作物的高通量SNP 数据的分析进行了讨论。 关键词 单核苷酸多态性, 作物, SNP 分型 SNP Genotyping Methods for Crops in Post-genomic Era Zhao Qiongyi 1 Li Xin 2 Zhou Degui 3 He Fei 4 Zhou Shaochuan 3 Luo Da 1* 1 National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032; 2 School of Life Science and Biotechnology, Shanghai JiaoTong University, Shanghai, 200030; 3 The Rice Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou, 510640; 4 Shanghai Biochip Co., Ltd; National Engineering Center for Biochip at Shanghai, Shanghai, 201203 * Corresponding author, dluo@ DOI: 10.3969/mpb.008.000125 Abstract Single Nucleotide Polymorphisms (SNPs) are the most widespread DNA sequence variation in organisms and widely applied in plant functional genomics and genetic improvement for crops. It is essential to use some feasible SNP genotyping approaches in assessing the quality of those data. For crops, it is important to identify and validate SNP markers that are needed for study in many fields, such as genetic variation, population structure analysis, as- sociation study, crop plants breeding, etc., This mini review focused on some commonly SNP genotyping methods, and gave a brief introduction to their detection principle and operatio