脂多糖预处理介导Nrf2对大鼠脊髓损伤的保护作用研究朱庆茂1蒋电.DOC

脂多糖预处理rf2对大鼠脊髓损伤 朱庆茂1，蒋孟 ［摘要］ 目的 探讨大鼠经低剂量脂多糖预处理LPS+EV)组、Nrf2干扰IV)组、LPS+Nrf2干扰LPS+NIV)组，每组llen’s打击法制备创伤性脊髓损伤（traumatic spinal cord injury,TSCI）basso beattie and bresnahan)评分。获取脊髓损伤段组织标本，HE染色观察其组织病理变化；Nissl染色观察神经元中尼氏小体变化及神经元生存指数；免疫组化及Western-Blot检测Nrf2，GCLC蛋白的表达0.05）IV组与LPS+NIV组之间评分无统计学差异（P＞0.05）与d时，LPS+EV组Nrf2蛋白表达明显增高（P＜0.05）Nissl染色显示神经元生存指数升高（P＜0.05）d，LPS+EV组GCLC蛋白表达增加（P＜0.05）其rf2/ARE/GCLC抗氧化应激通路来实现的。 ［关键词］ 脊髓损伤；脂多糖；预处理；氧化 ［中图分类号］ R651.2 ［文献标识码］ A Low dose LPS preconditioning activate Nrf2 promote GCLC in rats with traumatic spinal cord injury Zhu Qingmao1，Jing Dianming，Meng Chunyang2，Li Weichao1 （1Department of Orthopedics，First Affiliated Hospital，Chongqing Medical Universty，Chongqing，400016；2Department of Orthopedics，Affiliated Hospital， Jining Medical College，Shandong，272000，China） Abstract］ Objective To investigate the neuroprotective?effect and possible mechanism on rats with low dose LPS preconditioning after spinal cord injury．Methods 120 female SD rats were randomly divided into the empty virus (EV) group，LPS+empty virus (LPS+EV) group，Nrf2 interference virus (NIV) group，LPS+Nrf2 interference virus (LPS+NIV) group．The model of traumatic spinal cord injury (TSCI) was established by the modified Allen’s method，motor function of the rat hind limb was assessed by the Basso Beattie and Bresnahan (BBB) score at 1，3，7，14 and 28d after the operation．The injured spinal cord tissue samples were harvested at each time，and the pathological changes of rat spinal cord were observed by HE staining，the Nissl body and neuron survival index were observed by Nissl staining，the expressions of Nrf2 and GCLC protein level were detected by immunohistochemical staining and Western blotting．Results The rat BBB score of LPS+EV group increased significantly than EV group at 7，14，28d after operation（P0.05）e NIV group between LPS+NIV group have no statistical significance at each time．As compared with empty virus group：the Nrf2 protein of LPS+EV group was expression increa