毛细管微萃取结合微顺序注射在线检测全血中异丙酚含量.PDF

毛细管微萃取结合微顺序注射在线检测全血中异丙酚含量 王庆振，李莉*(新疆医科大学，乌鲁木齐 830011) 摘要：目的 建立一种快速测定全血中异丙酚含量的原位、在线分析的新方法。方法 利用毛细管微萃取器联合紫外光纤 传感仪及微顺序注射- 阀上实验室系统在线测定全血中异丙酚含量。结果 该方法的加样回收率为 95.8%~103.6%；低、 中、高 3 个浓度(100 ，150，200 μg·mL1)的日内精密度 RSD 分别为 7.2% ，8.2%和 2.6% ，日间精密度RSD 分别为 10.0%， 6.1%和 7.7%。与传统液液萃取方法相比，该方法异丙酚平均萃取率提高 3.5% ，萃取时间缩短5~7 min。结论 该方法样品 前处理无需离心、除蛋白等操作, 能快速测定全血中异丙酚的含量, 为全血中异丙酚含量原位、在线检测提供了良好的手段。 关键词：异丙酚；光纤化学传感；毛细管微萃取器；微顺序注射- 阀上实验室系统 中图分类号：R927.2 文献标志码：B 文章编号：1007-7693(2016)01-0016-04 DOI: 10.13748/ki.issn1007-7693.2016.01.004  Online Determination of Propofol in Whole Blood by Combining Capillary Micro-extraction with Micro-sequential Injection * WANG Qingzhen, LI Li (Xinjiang Medical University, Urumqi 830011, China ) ABSTRACT: OBJECTIVE To determine the propofol’s content in whole blood online and in situ by combined capillary micro-extraction rapid extraction with optical-fiber. METHODS Capillary micro-extractor, optical-fiber sensing devices and microSIA lab-on-valve were used to determine the propofol in whole blood. RESULTS The new methods of recovery ranged in 95.8%103.6%. The intra-day RSDs were 7.2%, 8.2% and 2.6%, and inter-day RSDs were 10.0%, 6.1% and 7.7%, respectively at 3 concentrations(100, 150, 200 μg·mL1). The average extraction rate was higher than tradition liquid-liquid extraction about 3.5%, and the extraction time was still shorter about 57 min. CONCLUSION This new method could test propofol in whole blood online and in situ without using centrifuge and removing protein, and provide an excellent opition to determine the propofol in whole blood. KEY WORDS: propofol; optical-fiber chemical sensor; capillary micro-extractor; microSIA lab-on-valve 异丙酚是一种新型全身静脉注射麻醉药，起