利用麻风树油驯化筛选高活性脂肪酶产生菌及其催化酯化反应.PDF

微生物学通报 DEC 20, 2009, 36(12): 1807~1811 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 利用麻风树油驯化筛选高活性脂肪 酶产生菌及其催化酯化反应 * 袁丽红 黄 晶 陆玉婷 周海霞 ( 210009) 摘 要: 以1株分解麻风树油的脂肪酶产生菌Pseudomonas sp. LP-1 为出发菌株, 通过麻疯树油定 向驯化筛选获得1 株酶活较高且产酶稳定的菌株P . sp. X -2-45, 其水解酶活为29.79 U/mL, 比原始 菌株提高了288% 。对P . sp. X -2-45 生长与产酶特征、对植物油脂水解能力及在有机相中催化脂肪 酸和有机醇间的酯化反应研究发现, 该菌株生长速率和产酶速率明显加快, 培养30 h 时生物量和 酶活达到最大, 稳定期延长, 培养过程中脂肪酶在培养基中的稳定性提高。以麻疯树油诱导合成 的P . sp. X -2-45 脂肪酶对麻疯树油的水解能力比原始菌株提高了378%, 说明采用麻风树油定向驯 化可提高脂肪酶对相应底物的水解能力。X -2-45 脂肪酶可以催化月桂酸与正丁醇、正辛醇、月桂 醇和丙三醇之间, 棕榈酸、硬脂酸与甲醇、正辛醇、月桂醇和丙三醇之间, 油酸与甲醇、正丁醇、 正辛醇、月桂醇和丙三醇之间发生酯化反应。 关键词: Pseudomonas sp., 脂肪酶, 驯化, 麻风树油, 酯化反应 Breeding of High Lipolytical Bacteria by Acclimation to Jatropha Oil and Resultant Strain-catalyzed Esterification Reaction * YUAN Li-Hong HUANG Jing LU Yu-Ting ZHOU Hai-Xia (College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, Jiangsu 210009, China) Abstract: A strain, Pseudomonas sp. X -2-45, with high and stable lipolytical activity was screened by con- tinuously subculturing a lipase-producing bacterium P. sp. LP-1 in culture medium containing Jatropha oil as a sole carbon source. Its hydrolytic activity was 29.79 U/mL, which was increased by 288% as compared to that of pare