荧光纳米材料应用于蛋白质分析的研究进展.pdf

※专题论述 食品科学 2015, Vol.36, No.05 221 荧光纳米材料应用于蛋白质分析的研究进展 王 琪，王蓓蓓，马美湖，蔡朝霞* （国家蛋品加工技术研发分中心，华中农业大学食品科学技术学院，湖北武汉 430070 ） 摘  要：蛋白质与人体新陈代谢、免疫应答和疾病发生等密切相关，因此对蛋白质研究是当今世界共同关注的热 点，目前已经渗透到生物、医学、食品等各个领域。纳米技术的发展极大地促进了蛋白质技术的研究，发展光学特 性优良、生物相容性好的功能化荧光纳米材料对深入研究蛋白质的标记、分析以及分离具有非常重要的意义。本文 主要介绍了半导体量子点、嵌入式核壳荧光纳米颗粒、碳点、碳纳米管、贵金属元素纳米簇和磁性荧光纳米颗粒等 荧光纳米材料的基本性质，以及各种荧光纳米材料在蛋白质标记成像、蛋白质相互作用、蛋白质固定与分离以及蛋 白质分析检测方面的应用。 关键词：荧光；纳米材料；蛋白质；应用 Recent Progress in Fluorescence Nanomaterial Application in the Field of Protein Analysis * WANG Qi, WANG Beibei, MA Meihu, CAI Zhaoxia (National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China) Abstract: Protein plays an important role in human metabolism, immunity and disease occurrence. At present, proteins have been studied in the fields of biology, medicine, food and others. The technologies used for protein research have become the focus of worldwide attention. Developing functional fluorescent nano-materials with good optical properties and biocompatibility is of great significance for the labeling, analysis and separation of protein. In this paper, the basic properties of fluorescent nano-materials are outlined, such as semiconductor quantum dots, embedded core-shell nanoparticles, carbon dots, carbon nanotubes, precious metal element nano-clusters, and fluorescent magnetic nanoparticles. We also review the applications of these kinds of nano-materials in protein marker imaging, protein interaction, protein immobilization and separation, and protein detection. Key words: fluorescence; nanometer materials; protein; application