不饱和脂肪酸对黄鳝生长及免疫指标的影响.pdfVIP

安徽农业大学学报，2008，35(2)：224—228 Journal of Anhui Agricultural University 不饱和脂肪酸对黄鳝生长及免疫指标的影响 杨鸢劫，邴旭文 ，徐增洪 (中国水产科学研究院淡水渔业研究中心，农业部水生动物遗传育种和养殖生物学重点开放实验室，无锡214081) 摘 要：按正交法L。(3 )在不含脂肪酸的基础饲料中，分别添加亚油酸(c 2n一6)、亚麻酸(C ：3n一3)、二 十碳五稀酸(c 5n一3，EPA)和二十二碳六稀酸(c 2：6n一3，DHA)，采用宝应湖野生黄鳝群体进行投喂饲养，数据 统计采用SPSS11．5软件进行分析。结果显示，饲料中添加不饱和脂肪酸对黄鳝增重率和增长率的第一限制因子 为Cl ：3n一3，其次是C 2n一6和 EPA+DHA；影响肥满度的主要因子是 C ：2n一6；对肝体指数产生一定影响的 是EPA+DHA；对成活率没有产生影响。血清溶菌酶(LSZ)活性有不同程度的提高，血细胞吞噬率和吞噬指数相关 系数达到显著水平，显著地增加了黄鳝非特异性免疫能力。由此得到黄鳝饲料中不饱和脂肪酸(UFA)C ：2n一6、 CIR：3n一3和EPA+DHA的最佳含量分别为1．30％、1．55％和0．25％。 关键词：黄鳝；不饱和脂肪酸；溶菌酶；吞噬活性 中图分类号：s966．4 文献标识码：A 文章编号：1672—352X(2008)02-0224-05 Effects of unsaturated fatty acids on the growth and immunity indices of Monopterus albus YANG Yuan—jie，BING Xu—wen，XU Zeng—hong (Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology，Ministry of Agriculture， Freshwater Fisheries Research Center，Chinese Academy of Fishery Sciences，Wuxi 214081) Abstract：The orthogonal diagram was applied to design the dose of additive according to the factors of linoleic acid，linolenic acid and EPA +DHA．The feedstuff contained no fatty acids as basic component and above additive were fed to Monopterus albus community from Baoying lake．The data on feedstuff was collected and analyzed by SPSS1 1．5．The result showed as follows：C ：3 n一3 was the first limiting factor to weight gain rate and increase rate ofMonopterus albus．C】8：2n一6 and EPA4-DHA were the inferior factors．C】8：2n一6 was the main factor to fat— ness．EPA+DHA had some effects on liver—somatic index though which had no effects on survival rate．The activity of lysozyme enhanced in some way．The correlation between phagocytic capacity of haemocyte and phagocytic indi— ces was significantly，improved the non—specific immunity of Monopterus albus si