中科院植物激素课件.pdf

第八章油菜素内酯 第一节 油菜素内酯的发现及结构 1 1. 发现 Pollen extract was found to promote cell and stem elongation (1941) POLLEN EXTRACT CONTROL Beans treated with pollen extract showed dramatic stem and cell elongation as compared to control bean. 2 • 1968年，日本名古屋大学的Marumo等从400 kg蚊母 树(Distylium racemosum Sieb et Zucc)树叶中分离 到751 g蚊母素A1和236 g蚊母素B，经过稻叶倾斜 法测试后，发现其引起稻叶弯曲的生物活性明显高于 IAA，他们认为这是由新的生长素引起的； • 但因为实验材料不足，又缺乏合适的分析仪器，未能 进一步分离并确定其结构，也未引起科学界的普遍关 注(Agric Biol Chem, 1968)； • 后来查明，这种物质就是油菜素内酯类物质。 3 The novel compound was partially purified and named “brassin” (1970) Control Gibberellin Brassinosteroid In 1970 Mitchell and colleagues showed that their newly purified compound promotes stem elongation. Mitchell, J.W., Mandava, N., Worley, J.F., Plimmer, J.R., and Smith, M.V. (1970). 4 Brassins - a new family of plant hormones from rape pollen. Nature 225: 1065-1066. The structure of brassinolide (BL) was identified (1979) 227kg Starting with pollen collected by bees, brassinolide was purified and its s