葡萄糖载体.docxVIP

葡萄糖载体 作者：张熙泉 张微微 黄勇华 田玉旺 【摘要】 目的 探讨血-脑屏障（BBB）中葡萄糖载体-1（55kDa Glut-1）在高血压性脑出血（HIH）病理进程中表达水平的变化规律及其发病关系。方法 人脑组织标本来自15例尸检HIH 患者和7例非神经系统疾病死亡的尸检作为对照。应用免疫组化方法（兔抗人的Glut-1抗体、鼠抗人的GFAP抗体）进行标记，分析脑出血4～18h、24～32h、72h后的脑BBB毛细血管内皮细胞中Glut-1蛋白表达水平的变化规律。同时，采用多媒体彩色病理图文系统（MPIAS-500）计算脑BBB-Glut-1的平均灰度（AGD，0～255）和平均光密度（ALD）。结果 对照组55kDa Glut-1 在脑微血管（主要是BBB毛细血管）内皮细胞特异性表达，显示清晰完整的内皮细胞边界。其AGD 、ALD均值分别是188.83±4.95和0.129±0.014。HIH 组，Glut-1 蛋白在出血病灶周围脑微血管内皮细胞的表达水平在4～18h组开始应激性增高，在24～32h组至高峰（其AGD、ALD 均值分别是156.49±10.37和0.220±0.042)，其中AGD显著低于对照组17.12%（P＜0.001），ALD显著高于对照组70.54%（P＜0.001）。HIH 72h之后组，脑组织广泛水肿、坏死，BBB破坏，55kDa Glut-1的表达已经明显下降低于对照组。结论 BBB-Glut-1（55kDa）作为研究BBB组织特异性基因表达的模型，在HIH患者脑组织中Glut-1即早应激性表达增加。证实了Glut-1基因在环境应激状态下具有诱导反应的特性，为充分保障脑的葡萄糖供应和正常维持脑的能量代谢稳态提供了一种重要的防御调控机制。 【关键词】 葡萄糖载体-1 血-脑屏障 高血压性脑出血 免疫组织化学 【Abstract】 Objective To explore the pathogenic relationship between glucose carrier protein 1 (55kDa Glut-1) in blood-brain barrier(BBB) and hypertensive intracerebral hemorrhage(HIH).Methods Human brain tissue samples were obtained at autopsy from 15 patients with HIH and 7 age-matched controls. We adopted immunohistochemical techniques (Ultra Vision Streptavidin/Peroxidase-plus Kit) and a rabbit anti-human Glut-1 polyclonal antiserum was used to investigate 55kDa Glut-1 immunoreactivity of cerebral microvessel endothelial cells ( largely in BBB) in three groups of HIH cases compared with controls，4～18h，24～32h and 72h later，according to the duration of inpatient to death.And using MPIAS-500，we calculated their immunostaining cells average grey degree ( AGD ) and average light density (ALD).Results Our study indicated 55kDa Glut-1 presented specificly at high concentrations in the BBB，AGD and ALD were (188.83±4.95) and (0.129±0.014) respectively. In the early acute insult periods with HIH，Glut-1 were enhanced expression in the BBB surrounding the hemorrhage lesions than that of the controls，significantly at 24～32h groups，ADG and ALD were (156.49±10.37) and (0.220±0.042)