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MTT升级版细胞计数试剂盒CCK8说明书
Revised August 16, 2002
Cell Proliferation Assay and Cytotoxicity Assay
Cell Counting Kit-8
Technical Manual
Product Code: CK04-11 (1000 tests)
CK04-13 (3000 tests)
Contents
General Information 1
Advantages 1
Storage 2
How to Use Cell Counting Kit-8 2
Required Equipments and Materials 2
Protocol 2
Cell Proliferation Assay 2
Cytotoxicity Assay 2
Background Control 2
Precautions 3
Frequently Asked Questions 3
References 3
Appendix 4
Cytotoxicity Assay Data 4
Product Code and Price 4
Related Products 4
General Protocol at a Glance 5
For the most up-to-date technical manual, access /tm
GENERAL INFORMATION As shown in Figure 3, the absorbance of the
CCK-8 assay solution is higher than that of MTT and
Cell Counting Kit-8 (CCK-8) allows very convenient other tetrazolium salts that produce water-soluble
assays by utilizing Dojindo’s highly water-soluble formazan dyes. Figure 4 shows that the cell
tetrazolium salt. WST-8 (2-(2-methoxy-4-nitrophenyl)-3- proliferation assay using CCK-8 correlates well with
3
(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, the [ H]-thymidine incorporation assay. Thus, the
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