IL_8在大鼠羊水栓塞发病机制中的实验研究.pdfVIP

1370 Chin J Lab Diagn,September,2010, Vol 14, No. 9 : 1007- 4287(2010) 09- 1370- 02 IL8 1 1 1 2* 刘云梅 , 战 云 , 王新云 , 卢英强 ( 1. , 132021; 2. , 130021) : (AFE) Wistar 30 , 20 , , : ( ) 10 10 10 , 60 1 , - 8( IL8) HE ( ) IL8 ( P 0. 01) , ( P 0.01) , ( P 0.05) IL8 , : ;8; ; : R392 :A The Experimental Study of IL8 in Rattus norvegicus AFE pathogenesis LI U Yunmei, ZHAN Yun, WANG Xinyun, et al. ( Dep artment of Ob teric and Gynecology , NO. 1 General H o p ital of Jilin Chemical Indu trial Group , Jilin 132021, China) Abstract: bjective The experimental study of AFE pathogenesis.Methods The afternoon gravidity Wistar Rattus norvegicus 30, the embryo age 20 days,According the difference of the liquid input, divides into3 groups stochastic: Comparison group (physio logical brine group) 10, amniotic fluid group10, embryo dung group 10.Preparation animal model. Observes 60 minutes and elicit the artery blood 1 mg afterwards to execute the animal.Inthe blood the IL8 content determination uses the ELISA,takes the lung organi zationuses HE coloration method to observe PMN in lung vein cavity expression. Results The experimental group( amniotic fluid group and embryo dung group) the IL8 content increase to comparison blood ( P 0.01) .In the experimental group lung vein cavity the PMN quantity increase to comparison group ( P 0.01) The embryo dung group and the amniotic fluid group compare also has difference ( P 0.05) . Conclusion In amniotic fluid inflammation allergy factor IL8and PMN to participate in the AFE occurrence development.the important factors of the cause of AFE. Key words:Amniotic Fluid Embolism; IL8;PMN; allergy